p53基因家族在人不同分化的肺鳞癌组织中的表达研究  

Expression of p53 gene fainilies in different differentiated squamous cell carcinoma in lung cancer tissue

作　　者：王志鹏[1] 张伟兵[1] 张清媛[2] 

机构地区：[1]哈尔滨医科大学附属第四医院VIP2病房,哈尔滨150001 [2]哈尔滨医科大学附属届肿瘤医院内三科

出　　处：《实用肿瘤学杂志》2010年第3期230-233,242,共5页Practical Oncology Journal

摘　　要：目的通过检测p53、△Np63和△Np73在肺鳞癌组织中的表达水平及与临床参数间的关系，探讨其在肺鳞癌发生、发展中的意义。方法应用免疫组化方法，在149例不同分化的肺鳞癌组织和39例癌旁组织中检测p53、△Np63和△Np73的表达水平．结果在肺鳞癌组织切片中p53、△Np63和△Np73蛋白阳性表达率分别为64．4％（96／149）、85．9％（128／149）和77．2％（115／149），均明显高于癌旁组织（P〈0．05）；p53、△Np63和△Np73在肺鳞癌组织中的表达与肿瘤分化程度有关（P〈0．05）；而与患者性别、年龄、临床分期、有无淋巴结转称等无关。结论p53、△Np63和△Np73是一组有价值的诊断肺鳞癌的指标，可能作为癌基因在肿瘤的发生机制中促进肿瘤的进展。Objective To investigate the expression of p53, △Np63 and △Np73 and their association with clinical pathology characteristics in different differentiated lung squamous ceil carcinoma. Methods Expression of p53,△Np63 and △Np73 was detected by immunehistoehcmistry in 149 cases of different differentiated lung squamous cell carcinoma and 39 cases adjaceni normal tissues. Results The positive rates of p53,△Np63 and △Np73 were 64.4% （96/149） ,85.9% （128/149） ,77.29（ （115/149） ,respectively in lun squamous cell carcinoma. The positive rates and expression level in primary lung squamous cell carcinooma were remarkably higher than that in adjacent normal tissues（ P 〈 0.05 ）. The expression of p53 ,ANp63 and ANp73 were not correlated with gender, age, clinical stage and mehtstasis in lymphoid nodes （ P 〉 0.05 ） , however they were correlated with the degree of tmnor differentiation（ P 〈 0.05 ）. Conclusion p53,△Np63 and △Np73 is a group of valuable diagnostic markers of lung squamous cell cancinoma, D53, △Np63 and △Np73 may be as oncogenes to promote the progression of tumor.

关 键 词：P53 △NP63 △Np73 肺鳞癌 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]