TGF-β1、TGF-βR1、Smad4在乳腺癌发生、发展中的作用及其相互关系  

Roles and relationships of TGF-β1,TGF-βR1 and Smad4 in the initiation and progression of breast cancer

作　　者：夏立平[1] 刘晓力[2] 陈国平[1] 郑武平[1] 何冬雷[1] 高炳玉[1] 

机构地区：[1]海南医学院附属医院,海南海口570102 [2]南方医科大学附属南方医院,广东广州510000

出　　处：《海南医学院学报》2010年第6期693-698,共6页Journal of Hainan Medical University

基　　金：海南医学院科研基金资助学报项目(0020100191)~~

摘　　要：目的:探讨TGF-β1、TGF-βR1及Smad4三者在乳腺癌中的表达与其发生、发展、转移等生物学行为以及预后的关系,为乳腺癌发病和治疗机制提供理论和实验依据。方法:提取30例乳腺癌临床标本(经液氮、研磨等处理)和MCF-7、T47D、SKBR-3三种乳腺癌细胞(取指数增长期细胞)总RNA,反转录cDNA第一链,克隆TGF-βR1、Smad4基因片段,测定质粒浓度,制备标准样品,并作出标准曲线,探针法荧光定量PCR(FQ-PCR)检测TGF-βR1和Smad4基因mRNA表达。结果:FQ-PCR检测发现恶性程度高的SKBR-3细胞,其TGF-βR1和Smad4的基因拷贝数明显低于恶性程度低的MCF-7细胞(P均<0.01),T47细胞两种基因拷贝数介于两者之间。结论:随着乳腺癌细胞病理类型恶性程度增加,TGF-βR1和Smad4 mRNA表达逐步下降,表明TGF-β1和Smad4可能具有类似乳腺癌抑癌基因的作用。Objective： To investigate the expression of TGF-β1,TGF-βR1 and Amad4 proteins and the relationship to the initiation,progression,metaptosis and prognosis of breast cancer.Methods： The samples of 30 cases and 3 cell lines（MCF-7,T47D,SKBR-3） of breast cancer were selected,and the total mRNA was extracted.Reverse transcription,and TGF-βR1 and Smad4 genes cloning were carried out.Then the concentration of plasmid was measured,standard curve was made,and the expression of TGF-βR1 and Smad4 mRNAs were assayed by FQ-PCR machine.Results： The gene copies of TGF-βR1 and Smad4 in SKBR-3 cells were lower than that in MCF-7 cells（P0.01） and the gene copies in T47 was middle.Conclusions： Our studies by FQ-PCR show that the lower TGF-βR1 and Smad4 mRNAs expression,the worse biology characteristic of breast cancer,which indicate TGF-βR1 and Smad4 act possibly as the role of anti-oncogene in breast cancer.

关 键 词：转化生长因子Β1 转化生长因子Β1受体 SMAD4 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]