超高效液相色谱-串联质谱法测定浓缩苹果汁中的熊果苷  

Determination of arbutin in apple juice concentrate by ultra performance liquid chromatography with electrospray ionization tandem mass spectrometry

作　　者：孔祥虹[1,2] 何强 乐爱山 吴双民 李建华 

机构地区：[1]陕西师范大学,陕西西安710062 [2]陕西出入境检验检疫局,陕西西安710068

出　　处：《色谱》2010年第6期632-634,共3页Chinese Journal of Chromatography

基　　金：国家质量监督检验检疫总局科研课题项目(No.2007IK166);陕西省科技计划项目(No.2008K01-06)

摘　　要：建立浓缩苹果汁样品中熊果苷的固相萃取-超高效液相色谱-串联质谱(SPE-UPLC-MS/MS)检测方法。浓缩苹果汁样品用水溶解、过滤后,用聚苯乙烯-二乙烯基苯共聚物(PS-DVB)固相萃取柱净化,外标法定量。测定时用Eclipse Plus C18色谱柱(100mm×2.1mm,1.8μm)分离,甲醇-水系统梯度洗脱;MS测定采用多反应监测(MRM)模式。熊果苷的检出限为0.02mg/L,在0.04～2.0mg/L的范围内标准溶液的峰面积与质量浓度呈良好的线性关系,回收率为75.2%～102.7%,相对标准偏差(RSD)低于8.9%。该方法简便、快速、灵敏,可用于浓缩苹果汁样品中熊果苷的检测和确证。An ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） method was developed for the determination of arbutin in apple juice concentrate.Samples were diluted with water,then cleaned-up with a PS-DVB column.Quantitation was carried out using an external standard method.UPLC was performed on an Eclipse Plus C18 column（100 mm×2.1 mm,1.8 μm） using a gradient solvent system（methanol-water）.MS/MS was performed with multiple reaction monitoring（MRM） mode.The detection limit of arbutin was 0.02 mg/L.The method showed good linear relationship at the range of 0.04-2.0 mg/L.The recoveries ranged from 75.2% to 102.7% with relative standard deviations（RSDs） less than 8.9%.The method is simple,fast and sensitive.It＇s suitable for quantitative and qualitative analysis of arbutin in apple juice concentrate.

关 键 词：超高效液相色谱-串联质谱 熊果苷 浓缩苹果汁 

分 类 号：O658[理学—分析化学]