簇毛麦低分子量谷蛋白基因克隆及序列分析  

Isolation and Characterization of LMW Glutenin Genes from Haynaldia villosa

作　　者：代寿芬[1] 颜泽洪[1] 

机构地区：[1]四川农业大学小麦研究所,温江611130

出　　处：《四川农业大学学报》2010年第2期119-124,共6页Journal of Sichuan Agricultural University

基　　金：四川省青年科技基金(09ZQ026-088);四川省教育厅重点项目(09ZA077)

摘　　要：以簇毛麦PI491576的总DNA为模板,经PCR克隆和测序得到了2个低分子量谷蛋白基因的完整编码区,暂定名为LMW-V1和LMW-V2,其核苷酸序列分别为810和855 bp,推导的氨基酸序列分别有270和285个残基。根据低分子量谷蛋白N端第一个氨基酸残基进行分类,这2个序列不属于LMW-m,LMW-s和LMW-i中的任何一种,为没有N端的新类型,其品质效应还尚待研究。序列比较显示,LMW-V1与长穗偃麦草的AAU43824序列一致性最高,达82%;LMW-V2与中间偃麦草的AAO53262一致性最高,达92%。系统进化分析表明,LMW-V1和LMW-V2与一些来自偃麦草E染色体组的低分子量谷蛋白和大麦H染色体组的B-hordein关系较近。Two low molecular weight glutenin（LMW glutenin） genes,designated as LMW-V1 and LMW-V2,were isolated and characterized by using genomic PCR from Haynaldia villosa PI491576.The DNA sequences of them were 810 and 855 bp,respectively.The corresponding amino acid lengths of them predicated by universal triplet codons were 270 and 285 residues,respectively.Based on the first residue in the N-terminal,these two genes could not be attributed to any of the LMW-m,LMW-s and LMW-i types,indicating that they were novel LMW glutenins that were not characterized so well to date.Sequence alignment indicated that LMW-V1 of Haynaldia villosa in the Triticeae tribe showed highest sequence identity with AAU43824 of Agropyron elongatum LMW glutenin,reaching 82%;while LMW-V2 showed the highest sequence identity with AAO53262 of Thinopyrum intermedium LMW glutenin,reaching 92%.Polygenetic tree based on the amino acid residues revealed that the LMW glutenins LMW-V2 and LMW-V2 of Haynaldia villosa had closer relationship with those genes from Lophopyrum and Barely B-Hordein genes than homologous genes from other Triticeae species.

关 键 词：簇毛麦 低分子量谷蛋白 基因克隆 序列分析 

分 类 号：S512[农业科学—作物学]