聚丙烯酰胺凝胶柱分离制备低聚果糖单组分  

Preparative separation of oligosaccharides monocomponent by polyacrylamide gel column

作　　者：王涛[1] 黄广君[1] 魏远安[1] 姚评佳[2] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]广西大学生命科学与技术学院,广西南宁530005

出　　处：《广西大学学报（自然科学版）》2010年第3期502-507,共6页Journal of Guangxi University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(29772006)

摘　　要：利用聚丙烯酰胺凝胶(Bio-Gel P-2)柱分离高纯度低聚果糖制备单一组分,结果表明,在1.6 cm×100 cm的玻璃柱上最佳的层析条件是洗脱速度0.2 mL/min、上样量0.5 mL(样液质量浓度0.4 g/mL)、柱温40℃。以Megazlaae公司的标准品为对照,经HPLC和MS分析,证明层析分离所得组分峰4、峰3和峰2分别是蔗果三糖、蔗果四糖和蔗果五糖。而且经HPLC面积归一化法定量分析,证明分离产品蔗果三糖、蔗果四糖、蔗果五糖蔗和果六糖的含量分别为99.91%、99.94%、99.33%和97.89%,表明其纯度很高,可以作为低聚果糖定量分析的标准品使用。The separation of high-purity fructooligosaccharide（FOS） monocomponents by polyacrylamide column was investigated.The results showed that optimized chromatographic conditions were as following： a flow rate of 0.2 mL/min,a sample volume of 0.5mL（o.4g/mL）,a column of 1.6×100 cm at temperature 40 ℃.The products obtained from the separation of FOS monocomponent（peak 5,4 3） were analysed by HPLC and MS using Megazlaae＇s standards as reference.The results indicated that the products included 1-kestose,1,1-kestotetraose and 1,1,1-kestopentaose,respectively.The purity of 1-Kestose to 1,1,1,1-kestohexose measured by quantitative analysis by HPLC peak area normalization were 99.91%,99.94%,99.33% and 97.89%,respectively.This indicated that the purity was high enough to be used as standard for quantitative analysis of fructooligosaccharides.

关 键 词：聚丙烯酰胺凝胶 分离 低聚果糖 蔗果三糖 蔗果四糖 蔗果五糖 

分 类 号：O629.12[理学—有机化学] O6-332[理学—化学]