浓缩富集+基因芯片快速检测水体中致病菌  

Pre-concentration + Gene Chip Detection Method Used for the Detection of Pathogens in Water Body

作　　者：王大勇[1] 肖进文 方振东[3] 王昱[2,4] 敖漉[1] 张春秀[5,6] 

机构地区：[1]后勤工程学院营房管理与环境工程系,重庆401311 [2]重庆进出口食品安全检测技术工程中心,重庆400020 [3]后勤工程学院,重庆401311 [4]重庆大学,重庆400030 [5]上海生物芯片有限公司,上海201203 [6]生物芯片上海国家工程研究中心,上海201203

出　　处：《后勤工程学院学报》2010年第4期65-70,91,共7页Journal of Logistical Engineering University

基　　金：重庆市科技攻关计划资助项目(CSTC,2008AB7132)

摘　　要：为了实现对水体致病菌的快速检测和监控，研究开发了一套预浓缩富集配合基因芯片检测的方法，对模拟和实际水样进行了检测，并与传统检测方法进行了比较。结果表明，通过对水样的浓缩集菌，基因芯片对致病菌的检出率（以肠炎沙门氏菌为单一检测对象）达到1．0×10^3cfu／mL，较原来提高了100倍；可以在8h内同步完成13种对人类有重大危害的致病菌的快速检测。Abstract In this study, a rapid pre-concentration ＋ gene chip detection method is developed for the detection and monitor pathogenic microorganism in natural water. After enrichment of pathogens by the Milliflex Plus concentrator,the detection rate of pure culture bacteria is 1.0×10^3 cfu/mL,which increases 100 times. At the same time,the detection duration reduces to 8 h. AS is observed in the research, the pre-concentration and gene mieroarray detection method can be one prosperous screen method for the detection of pathogenic microorganism pollution in the natural water body.

关 键 词：水体致病菌 浓缩富集 基因芯片检测 

分 类 号：X172[环境科学与工程—环境科学]