肾皮质细胞膜TGFβ受体在大鼠糖尿病发病中的作用  

Role of Transforming Growth Factor β Receptor in Renal Cortex from Diabetic Rats

作　　者：吴永贵[1] 周江华[1] 林善锬[1] 

机构地区：[1]上海医科大学华山医院肾内科

出　　处：《上海医科大学学报》1999年第1期37-40,共4页Journal of Fudan University(Medical Science)

基　　金：美国中华医学基金;国家自然科学基金

摘　　要：目的研究肾皮质细胞膜转化生长因子β（ＴＧＦβ）受体（ＴβＲ）在大鼠糖尿病发病中的作用。方法观察单侧肾切除后建立的糖尿病大鼠４周后血糖、血胰岛素、血肌酐水平、体重、肾重、肾组织蛋白含量及血浆、肾皮质、髓质ＡＣＥ活性的变化；利用ＲＴ－ＰＣＲ检测肾皮质中ＴβＲⅠ、ⅡｍＲＮＡ表达，Ｗｅｓｔｅｒｎ杂交检测肾皮质细胞膜ＴβＲⅠ蛋白表达。结果同对照组相比，糖尿病大鼠尽管体重进行性下降，肾重和肾组织蛋白含量却明显增加，肾皮质中ＡＣＥ活性也明显增加。ＲＴ－ＰＣＲ检测肾皮质内ＴβＲⅠ、ⅡｍＲＮＡ表达分别增加１．１２和４．０倍；Ｗｅｓｔｅｒｎ杂交显示肾皮质细胞膜ＴβＲⅠ蛋白表达增加１．１０倍。结论糖尿病大鼠肾皮质中ＴＧＦβＲ表达明显上调，可能是高糖和局部ＲＡＳ活性增加共同作用的结果。Purpose To study role of transforming growth factor β receptor(TβR)in the renal cortex from diabetic rats. Methods Blood glucose,serum insulin,serum creatinine,body weight,kidney weight and renal protein content as well as ACE activity in the plasma,renal cortex,medullawere observed after 4 weeks of SRZ injection.mRNA expression of TβRⅠ and Ⅱwere measured by quantitative reverse transcription polymerase chain reaction(RT-PCR);Protein expression of TβRⅠ was determined by western blot analysis in kidney cortex cell membrane. Results Diabetic rats developed hyperglycemia ( P <0.01),decreased serum insulin ( P <0.01),elevated serum creatinine ( P <0.05),body weight loss ( P <0.01),kidney hypertrophy ( P <0.05) and increased ACE activity ( P <0.05) in renal cortex.These changes were associated with 1.12 and 4.00 fold increase in TβRⅠ and Ⅱ mRNA expression,respectively.Protein expression of TβRⅠ was increased by 1.10 fold in renal cortex cell membrane. Conclusions Up-regulation of expression of TGFβR in renal cortex may be associated with hyperglycemia and locally active RAS.

关 键 词：糖尿病肾病 糖尿病 TGFΒ TΒR 

分 类 号：R587.2[医药卫生—内分泌] R692.02[医药卫生—内科学]