Ang-1治疗大鼠糖尿病视网膜病变的实验研究  

The Study of the Treatment for Diabetic Retinopathy in Rats by Ang-1

作　　者：陈桦[1] 曾莉[2] 

机构地区：[1]南昌大学研究生院医学部,2007级南昌330006 [2]南昌大学第一附属医院眼科,南昌330006

出　　处：《南昌大学学报（医学版）》2010年第2期7-11,F0003,共6页Journal of Nanchang University：Medical Sciences

基　　金：江西省自然科学基金(0640087)

摘　　要：目的观察玻璃体腔注射血管生成素-1(Ang-1)对糖尿病大鼠视网膜新生血管形成及血管内皮细胞生长因子(VEGF)的影响,并初步探讨其作用机制。方法选取健康SD大鼠60只,随机抽取其中15只作为正常对照组,不予处理。另外45只行左下腹腔注射链尿佐菌素(STZ)建立糖尿病模型,喂养3个月后分别行眼底血管荧光造影检查确立大鼠出现糖尿病视网膜病变(DR)即为成模。将成模大鼠再次随机分为DR对照组和Ang-1治疗组(每组15只)。DR对照组行双眼玻璃体腔注射PBS缓冲液5μL,Ang-1治疗组双眼玻璃体腔注射5μL浓度为160 mg.L-1的Ang-1。3 d后重复上述处理1次,继续观察3 d后处死各组大鼠,摘除眼球,取出视网膜行免疫组化检测比较VEGF的表达;常规病理切片HE染色比较突破内界膜且与内界膜有联系的内皮细胞核数。结果 DR对照组、Ang-1治疗组VEGF的表达与正常对照组比较明显增加(P<0.05),Ang-1治疗组VEGF表达比DR对照组明显减少(P<0.05)。DR对照组、Ang-1治疗组突破内界膜细胞数比正常对照组明显增加,而Ang-1治疗组新生血管细胞核数较DR对照组明显减少(P<0.05)。结论玻璃体腔注射Ang-1能明显减低VEGF的表达,减少视网膜新生血管芽的生成;其抑制糖尿病大鼠眼底新生血管的生成及渗漏可能是通过抑制VEGF的表达而发挥作用。Objective To observe the effect of angiopoietin-1（Ang-1） on diabetic rat retinal neovascularization and vascular endothelial growth factor（VEGF） by the vitreous cavity injection,and to explore its mechanism of action.Methods Selected 60 healthy SD rats,15 of them were randomly selected as normal control group and were not treated.Another 45 were injected of streptozotocin（STZ） through lower left abdominal for the establishment of diabetes model.Feeding after 3 months,checked the establishment of diabetic retinopathy in rats by fundus fluorescein angiography.Then,the rats were randomly divided into two groups,DR control group（n=15） and the Ang-1 treatment group（n=15）.The rats in DR control group were injected phosphate buffered saline（PBS） 5 μL through the vitreous cavity,Ang-1 treatment group were injected Ang-1 5 μL of a concentration of 160 mg·L-1.Three days later,repeat the above treatment once again.Continue to monitor three days,rats in each group were sacrificed and the eyes were resected,and detected the expression of VEGF in each group retina by immunohistochemical.Comparing the number of endothelial cells by conventional HE staining,which were breakthrough the internal limiting membrane and were associated with it.Results The VEGF expression levels of DR control group and the Ang-1 treatment group were significantly increased compared with that in normal control group（P0.05）,and the VEGF expression level of Ang-1 treatment group was significantly reduced compared with the DR control group（P0.05）.The number of breakthrough internal limiting membrane cells of DR control group and the Ang-1 treatment group were significantly increased compared with the normal control group.In Ang-1 treatment group compared with DR control group,the number of neovascular nuclei significantly reduced（P0.05）.Conclusion Ang-1 could significantly reduce the expression of VEGF by the injection through vitreous cavity,and reduce the generation of retinal neovascularization bud.Its inhibition

关 键 词：血管生成素-1 血管内皮细胞生长因子 糖尿病视网膜病变 动物 实验 大鼠 

分 类 号：R-332[医药卫生]