乙酰肝素酶mRNA在直肠癌组织中的表达及其临床意义  

Expression and Clinical Significance of Heparanase mRNA in Rectal Cancer

作　　者：周怀龙[1] 黎才海[2] 李其云[3] 易波[3] 

机构地区：[1]南昌大学研究生院医学部,2007级南昌330006 [2]江西护理职业技术学院党委办公室,南昌330029 [3]江西省肿瘤医院腹部外科,南昌330029

出　　处：《南昌大学学报（医学版）》2010年第2期40-43,47,共5页Journal of Nanchang University：Medical Sciences

摘　　要：目的研究乙酰肝素酶(Hpa)mRNA在直肠癌组织中的表达,探讨其与直肠癌分化、转移及预后的关系。方法采用RT-PCR方法检测40例直肠癌组织、癌旁正常组织、转移淋巴结、正常肠黏膜组织及6例直肠绒毛状腺瘤组织中Hpa mRNA表达情况,并分析其与直肠癌临床各病理参数的关系。结果 40例正常肠黏膜组织及6例直肠绒毛状腺瘤组织中Hpa mRNA的表达均阴性。40例直肠癌标本中27例Hpa mRNA表达阳性阳性表达率67.5%明显高于正常肠黏膜组织、癌旁正常组织及直肠绒毛状腺瘤组织(均P<0.01);直肠癌直径≥5 cm者HpamRNA阳性表达明显高于直径<5 cm者(P<0.05);有淋巴结转移者明显高于无淋巴结转移者(P<0.05);远处转移者高于无远处转移者(P<0.05);Ⅲ/Ⅳ期者表达明显高于Ⅰ/Ⅱ期者(P<0.05)。Hpa mRNA与患者的性别、年龄、肿瘤细胞分化无明显关系(均P>0.05)。结论 Hpa mRNA参与了直肠癌的生长、进展,并可能对直肠癌的浸润、转移有促进作用,可以作为反映直肠癌生物学行为的有效指标之一。Objective To study the expression of heparanase（Hpa）mRNA in rectal cancer and it＇s relationship with differentiation,metastasis and clinical prognosis.Methods RT-PCR was used to detect the expression of Hpa mRNA in 40 rectal cancer tissue,adjacent normal tissue,metastatic lymph nodes,distant normal mucosa and 6 rectum adenoma,then its relationship with clinicopathological parameters was analyzed.Results By RT-PCR detected,40 cases of normal mucosal tissues and the six cases of colorectal villous adenomas showed negative Hpa mRNA expression;40 patients with paired samples in 27 patients with rectal cancer Hpa mRNA expression was positive,positive expression rate was 67.5%,and the positive expression rate was significantly higher than that in normal mucosa,adjacent normal tissue and rectal villous adenoma（all P0.01）;the expression level in rectal diameter of ≥ 5 cm group was significantly higher than that in diameter 5 cm group（P0.05）,significantly with lymph node metastasis than in those without lymph node metastasis（P0.05）;metastatic lymph nodes was significantly higher than those without lymph node metastasis（P0.05）;distant metastasis group was higher than non-distant metastasis,Ⅲ/Ⅳ expression was significantly higher than that in patients with stage Ⅰ/Ⅱ persons（all P0.05）,while Hpa mRNA expression has no significant relationship with the patient＇s sex,age and tumor cell differentiation（all P0.05）.Conclusion Hpa mRNA may participate in the growth and progress of rectal cancer and may be involed in tumor invasion and metastasis,which could be regarded as one of the effective indicator to reflect the biological behavior of rectal cancer.

关 键 词：乙酰肝素酶MRNA 直肠癌 RT-PCR 临床意义 

分 类 号：R735.37[医药卫生—肿瘤]