X线照射诱导人胃癌SGC-7901细胞生物特性改变以及YKL-40基因表达变化  

Changes of biological characteristics and expression of YKL-40 gene in gastric cancer SGC-7901 cell induced by X-ray irradiation

作　　者：罗宏涛[1] 车团结[2] 王小虎[1] 庞春艳[3] 徐华[2] 陈卫[3] 李琳[2] 

机构地区：[1]甘肃省肿瘤医院放疗科,兰州730050 [2]兰州大学生命科学院细胞生物学研究所,730000 [3]兰州大学基础医学院生物化学与分子生物学研究所,730000

出　　处：《临床肿瘤学杂志》2010年第6期498-501,共4页Chinese Clinical Oncology

基　　金：甘肃省科技厅创新团队资助项目(098TTCA009)

摘　　要：目的观察X线照射后胃癌SGC-7901细胞形态改变、细胞周期分布情况以及是否可以诱导YKL-40基因的表达。方法将胃癌细胞株SGC-7901分为空白对照组(不照射)和实验组(2Gy/1f和4Gy/2f),实验组细胞经6-MVX线照射后取两组细胞在倒置显微镜下观察细胞形态学的改变,流式细胞术检测SGC-7901细胞周期的变化情况,半定量RT-PCR检测基因YKL-40mRNA的表达。结果实验组(2Gy/1f)细胞经X线照射后出现明显的形态学改变;流式细胞检测发现,与空白对照组细胞比较,2Gy、4Gy实验组细胞S期上调,G2/M期下调,组间比较差异有统计学意义(P<0.05)。RT-PCR检测胃癌细胞株SGC-7901不表达YKL-40或检测不到YKL-40mRNA,经X线照射后YKL-40表达。结论 X线照射可使人胃癌细胞发生形态改变及细胞增殖周期再分布,并且可诱导人胃癌细胞表达YKL-40。Objective To observe the morphological changes,the division of cells cycle and expression of YKL-40 gene level in gastric cancer SGC-7901 cells after irradiation by X-ray.Methods SGC-7901 cells were devided into control group（no irradiation）and experimental group（2Gy/1fraction and 4Gy/2fraction）.SGC-7901 cells in the two groups were taken.Morphological changes of the tumor cells by invert microscop,divisions of cell cycle by FCM and changes of YKL-40 mRNA of SGC-7901 cells by RT-PCR assay in experimental grop were observed compared to control group.Results Morphology of SGC-7901 cells in 2Gy/1 fraction group showed significant changes after irradiation.Cell S phase up and G2/M phase down in 2Gy/1 fraction group and 4Gy/2 fraction group,there was significant difference between the two groups（P〈0.05）.Meanwhile,expression of YKL-40 mRNA could be induced by irridiation.Conclusion We find that human gastric cancer SGC-7901 cells changed in morphology and redistributed in cell cycle after irradiation of X-ray.Inaddition,it can increase the expression of YKL-40.

关 键 词：胃癌细胞系 X射线 生物特性 YKL-40基因 

分 类 号：R735.2[医药卫生—肿瘤]