JWA基因对肿瘤细胞P-糖蛋白的表达及其功能的影响  

Effects of JWA gene on expression and function of P-glycoprotein in tumor cells

作　　者：许文侠[1,2] 张燕[1,2] 田甜[1,2] 田婷婷[1,2] 李爱萍[1] 周建伟[1] 徐珊[1,2] 

机构地区：[1]南京医科大学肿瘤中心,江苏南京210029 [2]南京医科大学细胞生物学系,江苏南京210029

出　　处：《中国肿瘤生物治疗杂志》2010年第3期292-296,共5页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金资助项目(No.30771829;30930080)~~

摘　　要：目的:研究JWA基因(又称ARL6IP5基因,ADP-ribosylation-like factor 6 interacting protein 5)对肿瘤细胞P-糖蛋白表达及其功能的影响。方法:利用脂质体转染技术将JWA-shRNA重组质粒及其空载质粒转入人绒毛膜癌细胞JAR和人乳腺癌细胞MCF-7,将Flag-JWA重组质粒及其空载质粒转入人绒毛膜癌耐依托泊苷(etoposide,VP16)JAR/VP16细胞;Westernblotting检测JWA和P-糖蛋白的表达,流式细胞术分析细胞内罗丹明123(Rhodamine 123,Rh123)的潴留情况。结果:JWA-shRNA重组质粒转入JAR细胞和MCF-7细胞后,JWA表达水平降低,P-糖蛋白表达水平上调,罗丹明潴留减少;Flag-JWA重组质粒转入JAR/VP16细胞后,JWA表达水平上调,P-糖蛋白表达水平下降,罗丹明潴留增加。结论:JWA基因可以调控肿瘤细胞P-糖蛋白的表达,并影响其转运功能。Objective To study the effects of JWA（ADP-ribosylation-like factor 6 interacting protein 5,ARL6IP5） gene on expression and function of P-glycoprotein in tumor cells.Methods： JWA-shRNA and control-shRNA plasmids were transfected into human choriocarcinoma cell line JAR and human breast cancer cell line MCF-7,and flag-JWA and flag-control plasmids were transfected into etoposide（VP16）-resistant JAR cells（JAR/VP16） by liposome-mediated transfection assay.JWA and P-glycoprotein expressions were examined by Western blotting analysis.Intercellular retention of rhodamine 123（Rh123） was determined by FCM.Results： After JWA expression was down-regulated by JWA-shRNA transfection,P-glycoprotein expression was increased in JAR and MCF-7 cells,and Rh123 retention was decreased.After JWA was over-expressed by flag-JWA transfection,P-glycoprotein expression was decreased in JAR /VP16 cells and Rh123 retention was increased.Conclusion： JWA gene can regulate the expression and transportation of P-glycoprotein in tumor cells.

关 键 词：JWA基因 P-糖蛋白 绒毛膜肿瘤 乳腺肿瘤 多药耐药 

分 类 号：R737.9[医药卫生—肿瘤] R730.5[医药卫生—临床医学]