PKC亚型选择性与辐射诱导肝HepG2细胞凋亡  

PKC isoform selectivity and radiation-induced apoptosis of HepG2 cells

作　　者：夏琼[1] 李传刚[1] 孙爱民[1] 张雪林[1] 

机构地区：[1]南方医科大学南方医院医学影像系,广东广州510515

出　　处：《南方医科大学学报》2010年第6期1376-1378,共3页Journal of Southern Medical University

摘　　要：目的探讨PKC在X线辐射诱导HepG2凋亡中发挥抗凋亡作用的PKC亚型选择性,进一步明确引起放射耐受的PKC亚型。方法 MTT法检测细胞存活率;HepG2细胞常规方法培养;细胞分对照组(不加任何处理因素)、辐射组(单纯6 Gy X线辐射);Varian2100直线加速器,6 MV X线,剂量6 Gy,剂量率为400 cGy/min辐射细胞;流式细胞仪检测PKC亚型的荧光强度和阳性细胞百分数及细胞凋亡率。结果辐射组HepG2细胞的PKCα平均荧光强度为2.28,与对照组细胞比较,差别具有显著统计学意义(P<0.05);辐射组细胞PKCδ平均荧光强度为5.03,与辐射前比较差别显著(P<0.01);而PKCζ、γ、ε、θ在两组细胞中平均荧光强度较低,且两组间差别不显著(P>0.05),未检测到PKCβ的表达。PKCα、δ的阳性细胞百分数在对照组及辐射组较其他所测亚型为高,且辐射亦使这两种PKC亚型的阳性细胞百分数升高;其他所测亚型阳性细胞百分数较低,且辐射对它们的影响不明显。结论 PKCα、δ可能在辐射诱导凋亡的保护作用占主导地位。Objective To investigate the expressions of protein kinase C（PKC） isoforms in X-ray-exposed HepG2 cells and identify the PKC isoforms that induce radioresistance in HepG2 cells.Methods Cultured HepG2 cells were divided into control group and 6 Gy radiation group for corresponding treatments.The fluorescence intensity（FI） and the percentage of positive cells were determined using flow cytometry.Results The FI of PKCα and PKCδ were 2.28 and 5.05 in the radiation group,respectively,significantly higher than those in the control group（P0.05）.The percentages of PKCα-and PKCδ-positive cells were significantly higher in the radiation group than in the control group（P0.05）.The FI and the percentages of PKC ζ,γ,ε,θ positive cells were rather low and showed no significant differences between the two groups （P0.05）;PKCβ expression was not detected in the two groups of cells.The apoptosis rates of the control and radiation groups were 1.73% and 20.90%,respectively.Conclusion PKCα and PKCδ may be involved in protecting HepG2 cells from radiation-induced apoptosis.

关 键 词：蛋白激酶C 辐射 亚型 

分 类 号：R363[医药卫生—病理学]