石菖蒲提取物及其初步分离物的抑菌活性研究  

Study on Inhibitory Activity of Traditional Chinese Drug Plant Acorus tatarinowii Schott. against Pathogens of Animals

作　　者：邓业成[1] 玉艳珍[1] 王萌萌[1] 张明[1] 骆海玉[1] 覃旭[1] 李丽芬[1] 

机构地区：[1]广西师范大学生命科学学院药用资源化学与药物分子工程教育部重点实验室,广西桂林541004

出　　处：《安徽农业科学》2010年第15期7836-7838,7875,共4页Journal of Anhui Agricultural Sciences

基　　金：药用资源化学与药物分子工程教育部重点实验室资助项目(桂科能07109001-11);广西自然科学基金资助项目(桂科自0991097);广西环境工程与保护评价重点实验室资助项目(桂科能0702K026)

摘　　要：[目的]研究石菖蒲对动物病原菌的抗菌活性。[方法]采用滤纸片法和带药培养基涂布法测定了中药植物石菖蒲茎和叶甲醇提取物对13种动物病原菌的抑菌活性;采用液-液萃取法对石菖蒲茎提取物的抑菌活性成分进行初步分离,并测定各萃取物的抑菌活性。[结果]用带药培养基涂布法测定,石菖蒲茎提取物对表皮葡萄球菌、藤黄微球菌、溶壁微球菌、蜡样芽孢杆菌、痢疾志贺氏菌、伤寒杆菌、巨大芽孢杆菌、金黄色葡萄球菌和枯草芽孢杆菌9种供试菌有较好的抑菌作用,其最低抑制浓度(MIC值)分别为1.5、2.5、3.5、2.0、1.5、2.5、2.5、3.5、2.5g/L,对变形杆菌、大肠杆菌、绿脓杆菌和白色念珠菌4种病原菌在样品浓度为10g/L时无抑菌活性;石菖蒲叶提取物的抑菌活性较差,在浓度为10g/L时,对痢疾志贺氏菌和蜡样芽孢杆菌在72h有抑菌活性,对其余供试菌无抑菌活性。用滤纸片法测定,结果与用带药培养基涂布法基本一致,但抑菌圈较小。用1.0g/L的各萃取物样品处理供试病原菌72h,正丁醇萃取物对9种供试病原菌有抑菌作用,石油醚萃取物、乙酸乙酯萃取物和水萃取物无抑菌作用。[结论]中药植物石菖蒲对多种动物病原菌具有抑菌活性,茎的抑菌活性高于叶,抑菌活性物质主要存在于正丁醇萃取物中。[Objective]The aim was to study inhibitory activity of traditional chinese drug plant Acorus tatarinwii Schott.against pathogens of animals.[Methods]The inhibitory activities of the methanol extracts from stems and leaves of traditional Chinese drug plant Acorus tatarinowii Schott.against 13 pathogens of animals were determined by filter paper disk method and culture medium coating method.The active ingredients of extract from stems of Acorus tatarinowii Schott.were preliminarily isolated by liquid-liquid extraction,and the inhibitory activity of the isolated petroleum ether extract,ethyl acetate extract,n-butyl alcohol extract and water extract against pathogens were determined.[Result]The results determined by culture medium coating method showed that the extract from the stems had inhibitory activity against Staphylococcus epidermidis,Micrococcus luteus,Micrococcus lysodeikticus,Bacillus cereus,Shigella dysenteriae,Salmonella typhi,Bacillus megaterium,Staphyloccocus aureus and Bacillus subtilis,with the MIC values of 1.5,2.5,3.5,2.0,1.5,2.5,2.5,3.5 and 2.5 g/L,respectively,and had no inhibitory activity against Proteus vulgaris,Escherichia coli,Pesudomonas aeruginosa and Monilia albican at 10.0 g/L;extract from leaves exhibited inhibitory activity only against Shigella dysenteriae and Bacillus cereus at 10.0 g/L after 72 h,while had no inhibitory activity against other tested pathogens.The results determined by filter paper disk method were approximately consistent with that determined by culture medium coating method,but the inhibition zones were smaller.With 1.0 g/L of each extract sample handling pathogens for 72 h,the results indicated that n-butyl alcohol extract had inhibitory activity against the 9 tested pathogens and other extracts showed no activity.[Conclusion]Traditional chinese drug plant Acorus tatarinowii Schott.had inhibitory activity against a variety of pathogens,inhibitory activity of stems was higher than leaves,and the active ingredients of Acorus tatarinowii Schott.mainly existed in n-but

关 键 词：石菖蒲 病原菌 抑菌活性 活性物质 

分 类 号：S567.239[农业科学—中草药栽培]