干扰素-γ对大鼠胚胎神经干细胞发育的影响  

The effect of IFN-γ on the growth and development of rat embryonic neural stem cells

作　　者：石蕊[1] 王玉贤[1] 

机构地区：[1]山西医科大学第一临床医院妇产科,太原030001

出　　处：《生殖医学杂志》2010年第3期250-255,共6页Journal of Reproductive Medicine

基　　金：山西省卫生厅科技攻关项目(200626)

摘　　要：目的探讨感染后细胞因子干扰素(IFN)-γ对胎鼠神经系统发育的影响。方法利用不同浓度IFN-γ(0、20、200、2,000 U/ml)对大鼠体外培养的胚胎16 d的神经干细胞进行刺激,观察神经干细胞生长状态、主要组织相容性复合体(MHC)表达和分化格局的变化。结果高浓度的IFN-γ(2,000 U/ml)对神经干细胞具有毒性作用。中等浓度的IFN-γ(200 U/ml)能够显著上调神经干细胞MHC-Ⅰ类和Ⅱ类分子的表达。同时,中等浓度的IFN-γ能够促进神经干细胞的分化,使得神经干细胞向神经元和少突胶质细胞分化增加,而向星形胶质细胞的分化降低。结论大剂量IFN-γ可改变神经干细胞的生长和分化状态,IFN-γ除了对胎儿神经系统具有直接毒性外,还可能通过影响MHC的表达引起母体对胎儿的排斥,也许对新生儿智力发育造成不利影响。Objective：To investigate the effect of IFN-γ on the development of rat embryonic neural stem cell （NSC）. Methods： Rat embryonic NSCs were isolated from rat fetuses on day 16 of gestation and cultured. IFN-γ at different concentrations （0, 20, 200 and 2,000 U/ml） was added into the culture medium. Immunohistochemistry was used to identify the cell types and flow cytometry to determine the expression of major histocompatibility complex （MHC） molecules. Results：The high dose of IFN-γ （2,000 U/ml）was toxic to embryonic neural stem cells. IFN-γ at dose of 200 U/ml could significantly up-regulate the MHC expression on NSCs and enhance the differentiation of NSCs to neurons and oligodendrocytes, but inhibit the differentiation of NSCs to astrocytes. Conclusions：The high dose of IFN-γ could alter the growth and differentiation of rat fetal NSCs. In addition to the direct toxicity to NSCs, IFN-γ is able to increase the MHC expression on NSCs to raise the risk of maternal immunorejection. The changes of differentiation pattern caused by IFN-γ will probably be harmful to baby＇s intelligence development.

关 键 词：发育 分化 干扰素-Γ 主要组织相容性复合体 神经干细胞 

分 类 号：R[医药卫生]