芸豆植物凝集素对昆明白小鼠精子畸形的影响  

Effect of phytohemagglutinin from Yunnan white kidney bean (Phaseolus Vulgaris) on sperm malformation in mice

作　　者：王昌梅[1] 张丽芬[1] 杨明洁[1] 张田[1] 王敏康[1] 

机构地区：[1]生物能源持续开发利用教育部工程研究中心云南师范大学生命科学学院,昆明650092

出　　处：《生殖医学杂志》2010年第3期262-265,共4页Journal of Reproductive Medicine

基　　金：国家自然科学基金资助(30560056;30860128);云南省中青年学术技术带头人后备人才培养计划(2005PY01);云南省教育厅科研基金资助(5Z0239B;06Y1168;07Z11378)

摘　　要：目的测定芸豆植物凝集素(PHA)对小鼠精子的影响。方法实验小鼠腹腔注射1/3 LD_(50),1/10 LD_(50)和1/30 LD_(50)的芸豆PHA,阴性对照组给予生理盐水,阳性对照组给予环磷酰胺,剂量为40 mg/kg体重。所有各组均按0.1 ml/只腹腔注射,每日一次,连续5 d。各组于首次给药后35 d和70 d取样制片观察。结果给药后35 d和70 d取材,高、中、低剂量组分别与阴性对照组相比,小鼠精子畸形率无显著差异(P>0.05),与阳性对照组相比,有极显著差异(P<0.01)。各剂量组之间相比,无显著性差异,即无剂量效应关系。结论 PHA并不引起小鼠精子畸形率升高。Objective： To test the effect of phytohemagglutinin （PHA） from Yunnan white kidney bean on sperm deformity in mice. Methods： Different doses of PHA at 1/3 LD50, 1/10 LD50 and 1/30 LD50 were injected into the abdominal cavity of mice, once daily for 5 consecutive days, with 0. 9% NaCl as negative control and cyclophosphamide at dose of 40 mg/kg b. w. as positive control. The injection volume was 0. 1 ml per animal. Epididymal samples were collected on the 35^th day and 70^th day after treatment and sperm smears were made for microscopic examination. Results： The sperm deformation rate in the mice treated with different doses of PHA was not significantly different from that of negative control at 35＇h day or 70th day of treatment, but significantly lower than that of positive control （P〈0.01）. There was no significant difference in sperm deformation rate among 3 different PHA dose groups. Conclusions： It can be concluded that there is no genetic toxicity of PHA on mouse sperm.

关 键 词：植物凝集素 精子畸形 小鼠 

分 类 号：R[医药卫生]