全脑缺血/再灌注大鼠海马PPARα表达变化  

Expression of PPARα mRNA and Protein in Hippocampi Following Global Cerebral Ischemia/Reperfusion in Rats

作　　者：张艳丽[1] 杨俊卿[1] 李咏[1] 庄瑞春[1] 潘永全[1] 

机构地区：[1]重庆医科大学药理教研室,重庆市生物化学与分子生物学重点实验室,重庆400016

出　　处：《四川动物》2010年第4期630-632,635,共4页Sichuan Journal of Zoology

基　　金：国家自然科学基金(30672211)

摘　　要：目的观察全脑缺血/再灌注损伤大鼠海马组织中PPARαmRNA和蛋白表达的动态变化。方法采用夹闭两侧颈总动脉,颈静脉抽血再回输建立大鼠全脑缺血/再灌注模型(I/R)。RT-PCR和Western Blot分别检测PPARαmRNA和蛋白在缺血再灌注不同时间的表达。结果大鼠海马PPARαmRNA表达在缺血/再灌注30min后升高,24h时达峰,而后降低,再灌注30d仍略高于正常水平。PPARα蛋白表达变化与PPARαmRNA表达相似。结论全脑缺血/再灌注损伤可诱导PPARα mRNA及蛋白表达,升高时限为30d。Objective To explore the changes of PPARα mRNA and protein expression in cerebral hippocampi of global cerebral ischemia reperfusion（I/R） rats. Methods The model of global cerebral ischemia reperfusion was established by bilateral common carotid arteries occlusion and vena cervicalis blood retransfusion. RT-PCR was performed to detect expression of PPARα mRNA. Western Blot was used to detect PPARα protein expression. Results The expression of PPARα mRNA in hippocampi increased at 30 min after global cerebral/JR, peaking at 24 h, and was still slightly higher than normal levels on the 30th day after I/R. The changes of PPARα protein expression were similar with that of PPARα mRNA expression. Conclusion Global cerebral I/R can induce an increase of expression of PPARα mRNA and protein in rat hippocampi within 30 days after insult.

关 键 词：PPARΑ 缺血/再灌注 脑损伤 

分 类 号：R741[医药卫生—神经病学与精神病学]