CA-125抗原、肿瘤抑制蛋白质p53、Ki-67抗原及抑癌基因p27蛋白作为卵巢癌病理辅助诊断指标的评价  

Diagnostic Value of Expression of CA125,P53,Ki-67 and P27 in Ovarian Carcinoma

作　　者：曾永群[1] 陈秋月[2] 李凡彩[2] 

机构地区：[1]桂林医学院附属医院妇产科,广西桂林市541001 [2]桂林医学院附属医院病理科

出　　处：《中国全科医学》2010年第17期1877-1879,共3页Chinese General Practice

摘　　要：目的评价CA-125抗原(CA125)、肿瘤抑制蛋白质p53(P53)、Ki-67抗原(Ki-67)及抑癌基因p27蛋白(P27)作为卵巢癌辅助诊断指标的临床意义。方法采用组织芯片微阵列技术检测所选免疫组化指标在96例卵巢癌和22例卵巢良性瘤组织中的表达,绘制受试者工作特征(ROC)曲线,并以病理诊断为金标准,比较单项和多项免疫检测指标联合检测的准确性。结果卵巢癌和卵巢良性瘤组织中CA125、P53、Ki-67、P27的阳性表达率比较,差异均有统计学显著意义(P<0.05)。P53、CA125、Ki-67、P27单项检测的AUC分别为0.730(P<0.01)、0.657(P<0.05)、0.617(P>0.05)和0.371(P>0.05);CA125+P53、CA125+P53+Ki-67、CA125+P53+Ki-67+P27联合检测使ROC曲线下面积(AUC)分别提高到0.773(P<0.01)、0.804(P<0.01)和0.824(P<0.01)。结论多个免疫组化指标联合检测均能不同程度地提高检测效能,优于任何单项检测。Objective To evaluate the diagnostic value of CA125,P53,Ki-67 and P27 expressions in ovarian carcinoma（OC）.Methods Tissue microarray technology was used to determine the expressions of selected immunohistochemical indicators in the tissues of 96 OC patients and 22 benign tumor patients.ROC curve was drawn.Taking pathological diagnosis as gold standard,the detection accuracy of single indicator or combined immune indicators was observed.Results There was significant difference in CA125,P53,Ki-67 and P27 expressions between groups ovarian cancer and innocent tumor（P〈0.05）.The areas under curve （AUC） detected singly by P53,CA125,Ki-67 and P27 were 0.730（P〈0.01）,0.657（P〈0.05）,0.617（P〉0.05）and 0.371（P〈0.05）,respectively,and AUC detected by combined CA125＋P53,CA125＋P53＋Ki-67 or CA125＋P53＋Ki-67＋P27 were 0.773（P〈0.01）,0.804 （P〈0.01） and 0.824（P〈0.01）,respectively.Conclusion Combined immunohistochemical indicators can improve the detection efficacy,superior to any single one.

关 键 词：卵巢肿瘤 CA-125抗原 肿瘤抑制蛋白质P53 KI-67抗原 P27蛋白 ROC曲线 诊断 

分 类 号：R737.31[医药卫生—肿瘤]