H22-pEGFP-C1-FL肝癌细胞瘤苗构建及其生物活性的研究  

The study of the construction of H22-pEGFP-C1-FL vaccine

作　　者：王书文[1] 和新盈[2] 李明众[1] 赵东利[1] 

机构地区：[1]西安交通大学第一附属医院肿瘤放疗科,陕西西安710061 [2]西安医学院病理教研室,陕西西安710068

出　　处：《现代肿瘤医学》2010年第7期1276-1278,共3页Journal of Modern Oncology

基　　金：陕西省科技攻关基金资助项目(编号:2003K10-G63)

摘　　要：目的:构建H22-pEGFP-C1-FL肝癌细胞瘤苗,研究其活体内应用的生物学活性。方法:通过G418筛选能高表达hFL蛋白H22-pEGFP-C1-FL的肝癌细胞株;小鼠尾静脉注射经放射线灭活的H22-pEGFP-C1-FL的肝癌细胞,检测注射后hFL蛋白表达情况及对骨髓抑制小鼠的升白作用。结果:获得高表达hFL蛋白的H22肝癌细胞。该细胞经辐射灭活后,输注给辐射后骨髓抑制的小鼠,与对照组相比,可快速升高白细胞(P<0.01),显著提高辐射小鼠的存活率(P<0.01)。结论:成功构建了H22-pEGFP-C1-FL肿瘤细胞瘤苗,为其临床应用奠定基础。Objective：To construct H22 -pEGFP- C1 -FL and detect the role of the anti -tumor effect both in vivo and in vitro. Methods：The recombinant plasmid pEGFP - C1 - FL was transformed to H22 cells, and Western - blot was employed to identify the expression of human Flt3 ligand. The efficacy of H22 - pEGFP - C1 - FL vaccine was detected on BALB/c mice, the number of peripheral blood cells was counted on irradiated mice after administrated H22 -pEGFP -C1 -FL vaccine. Results：Wenstern -blot showed the FL protein could be recognized by anti - FL antibody. After injection of the plasmid pEGFP - C1 - FL into mice tail vein with large volume within short period of time, H22 - pEGFP - C1 - FL vaccine used before irradiation could stimulate the recovery of peripheral white blood cells, significantly enhanced survival rate of irradiated mice. Conclusion：H22 -pEGFP- C1 -FL vaccine was suc- cesshtlly constructed.

关 键 词：氨酸激酶受体3的配体 造血因子 瘤苗 

分 类 号：R73-362[医药卫生—肿瘤]