CDH1基因启动子甲基化与宫颈癌临床病理特征的关系  

Promoter methylation of CDH1 genes in cervical cancer:correlation with clinicopathologic characteristics

作　　者：陈勇[1,2] 郑蓉[2] 曹桂荣[2] 赵昌银[2] 冯景[3] 陈双郧[2] 

机构地区：[1]湖北中医学院中医系 [2]郧阳医学院附属太和医院妇科 [3]郧阳医学院附属太和医院检验科,湖北十堰442000

出　　处：《现代肿瘤医学》2010年第7期1394-1397,共4页Journal of Modern Oncology

基　　金：湖北省教育厅自然科学重点项目(编号:b200724002);湖北省教育厅中青年人才项目(No:Q20082407);湖北省杰出青年人才项目(No:2008ABC042);湖北省自然基金项目(No:2007ABA371)

摘　　要：目的:检测Cadherin 1(CDH1)基因启动子区CpG岛甲基化状态,并分析CDH1基因甲基化率与临床病理特征的关系。方法:运用甲基化特异性PCR(MSP)检测95例宫颈癌标本及对照组40例正常宫颈组织的CDH1基因启动子甲基化状态,荧光定量PCR检测高危型HPV DNA。分析CDH1基因甲基化状态与不同临床病理特征之间的联系。结果:宫颈癌组CDH1基因甲基化率明显高于正常对照组(分别为49.5%,12.5%,P<0.01)。宫颈癌CDH1基因甲基化检测和高危型HPV DNA检测结果有较好的一致性(Kappa=0.332,P<0.001)。鳞癌组甲基化率较腺癌组高(分别为66.2%,7.4%,P<0.01)。CDH1甲基化阳性率随肿块增大及FIGO分期升高而增高(P<0.05)。不同年龄、肿瘤浸润深度及有无淋巴结转移和CDH1基因甲基化未见明显关系(P>0.05)。结论:宫颈癌CDH1基因启动子甲基化与高危型HPV具有良好的一致性。CDH1基因启动子高甲基化为频发事件,并与不同宫颈癌病理类型相关,可作为宫颈癌诊断及分型的生物学标志物。Objective：To investigate the promoter methylation status of CDH1 genes in cervical cancer and its correlation with clinicopathologic characteristics. Methods： Promoter methylation was evaluated using methylation - specific polymerase chain reaction （MSP） in 95 cervical cancer tissue specimens and 40 normal controls. The relationship between clinicopathologic parameters and methylation status was evaluated. Results： The frequencies of promoter methylation of CDH1 in cervical cancer were 49.5%. Cervical cancer had significantly higher methylation frequencies than that of the controls （P 〈 O. O1 ）. Methylation analysis of CDH1 promoter and high risk HPV DNA testing had good consistency（ Kappa = 0. 332, P 〈 0. 001 ）. The promoter methylation of CDH1 was significantly higher in squamous cell carcinoma （SCC）than in adenocarcinoma（AC） （66.2% vs 7.4% ,respectively,P 〈0.05）. The present promoter methylation frequency for CDH1 identified significant differences associated with tumor size, FIGO stages and WHO grades （ P 〈 0.05）. There were no significant associations between the methylation frequencies for CDH1 and age,invasion depth and lymph node metastasis. Conclusion： Promoter methylation of CDH1 and high -risk HPV DNA testing have good consistency. CDH1 gene promoter methylation is a frequent epigenetic event in cervical carcinoma and has a significant correlation with cancer pathological types, therefore can be used as cancer marker for diagnosis.

关 键 词：宫颈癌 CDH1基因 甲基化 MSP HPV 

分 类 号：R737.33[医药卫生—肿瘤]