玉米dbf1基因与耐旱相关性状的关联分析  被引量:14

Association Analysis between dbf1Gene and Drought-Tolerant Traits in Maize

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作  者:苏治军[1] 郝转芳[1] 谢传晓[1] 李明顺[1] 石红良[1] 吴永升[1] 张世煌[1] 李新海[1] 

机构地区:[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081

出  处:《植物遗传资源学报》2010年第4期474-478,482,共6页Journal of Plant Genetic Resources

基  金:国家自然科学基金(30600394;30721140554)

摘  要:脱水响应元件结合因子(DBF1蛋白)是高等植物体内DRE/CRT顺式元件的结合因子,是水分胁迫信号传导的核心构件。本文以玉米自交系郑22为材料,采用PCR技术分离得到dbf1基因区域基因组DNA(gDNA)全长949 bp,起始密码子至终止密码子序列长654 bp。该基因由1个外显子组成,无内含子。dbf1基因在175份玉米自交系中共检测到9个SNP变异(平均每57.3 bp一个),没有检测到In/Del变异,在3个多态性位点间存在较高程度的连锁不平衡(r2>0.5)。在干旱胁迫下,dbf1基因中的多态性位点397与ASI和单穗粒重显著关联(P<0.05),位点477与单穗粒重和结实株数百分率存在显著关联(P<0.05)。位点397对ASI和单穗粒重的贡献率分别为6.94%和10.03%;位点477对单穗粒重和结实株数百分率的贡献率分别是1.01%和3.66%。根据2个位点将175份自交系分成4种单体型,其中单体型4含有能使ASI减小、结实株数百分率增加的等位基因位点,包含早49、丹598、丹黄02等耐旱和中度耐旱自交系,推测单体型4可能是dbf1基因的耐旱单体型。研究结果为基于dbf1因开发耐旱功能标记提供信息。DBF1(DRE binding factor)protein encoded by dbf1 gene,is a cis-element of DRE/CRT and the core element of in vivo signal transmission under water stress in higher plant.The genomic sequence information of dbf1 gene is the premise to study the following natural variations of functional alleles and develop molecular marker for assisting breeding.In the present study,949bp of genomic region of dbf1 was sequenced using a maize inbred line Zheng 22 with PCR technology.The coding region of this gene was comprised of only one exon with a length of 654bp from promoter to terminator.Nine SNPs in dbf1 gene were identified among 175 maize inbred lines,and no IN/DEL(insertion and deletion)was found.Averagely,there was a variation every 57.3bp.Higher LD degree(Linkage disequilibrium,r20.5)was identified between three polymorphic site pairs.Two SNP sites in dbf1 gene were identified significantly to be associated with three traits(P0.05),ASI(Anthesis to silking interval),kernel weight per ear and ear setting percentage.Among them,nucleotide variation T/C on site 397 was significantly associated with traits ASI and kernel weight per ear with phenotypic contributions of 6.94% and 10.03%,respectively.Nucleotide variation A/G on site 477 was significantly associated with traits ear setting percentage and kernel weight per ear with phenotypic contributions of 1.01% and 3.66%,respectively.Based on both two sites,four haplotypes were identified among 175 maize inbred lines.Haplotype 4 by decreasing ASI and increasing ear setting percentage was thought to be superior candidate drought-tolerant haplotype,which was displayed in some drought-tolerant and medium tolerant inbred lines such as Zao 49,Dan 598,Danhuang 02,etc.The results provide basic information for analyzing the key candidate genes associated with drought tolerance under different water supplying levels.

关 键 词:玉米 dbf1 单核苷酸多态性 关联分析 单体型 

分 类 号:S513[农业科学—作物学]

 

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