Diversity of microbial community at acid mine drainages from Dachang metals-rich mine, China  

作　　者：周智君 尹华群 刘毅 谢明 邱冠周 刘学端 

机构地区：[1]School of Minerals Processing and Bioengineering,Central South University [2]Key Laboratory of Biometallurgy of Ministry of Education,Central South University

出　　处：《Transactions of Nonferrous Metals Society of China》2010年第6期1097-1103,共7页中国有色金属学报（英文版）

基　　金：Projects(50321402, 30428014, 50621063) supported by the National Natural Science Foundation of China;Project(2004CB619201) supported by the National Basic Research Program of China

摘　　要：Two acid mine drainage(AMD)samples TS and WK,which were from the Dachang metals-rich mine in Guangxi province,China,were studied using PCR-based cloning approach.A total of 44 operational taxonomic units(OTUs)were obtained from the two AMD samples.However,only three OTUs(GXDC-9,GXDC-19 and GXDC-50)detected in sample TS can also be observed in sample WK.Phylogenetic analysis revealed that the bacteria in the two samples fell into four putative divisions,which were Nitrospira,Alphaproteobacteria,Gamaproteobacteria,and Acidobacteria.Organisms of genuses Acidithiobacillus and Leptospirillum,which were in gamaproteobacteria class and Nitrospira family,were dominant in two samples,respectively.In sample TS,which was characterized by low pH,high sulfate,high iron,and high arsenide,two species(Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans)constituted 98.22% of the entire microbial community.Compared with sample TS,the microbial community in sample WK was more diversified according to the observation.Interestedly,the Legionella species,which was rarely observed in the low-pH environment,was detected in sample WK.This work helps us to further understand the diversity of microbial community living in extreme acid mine drainages with unique geochemistry and the tolerance capability of acidophiles to heavy metal.Two acid mine drainage（AMD）samples TS and WK,which were from the Dachang metals-rich mine in Guangxi province,China,were studied using PCR-based cloning approach.A total of 44 operational taxonomic units（OTUs）were obtained from the two AMD samples.However,only three OTUs（GXDC-9,GXDC-19 and GXDC-50）detected in sample TS can also be observed in sample WK.Phylogenetic analysis revealed that the bacteria in the two samples fell into four putative divisions,which were Nitrospira,Alphaproteobacteria,Gamaproteobacteria,and Acidobacteria.Organisms of genuses Acidithiobacillus and Leptospirillum,which were in gamaproteobacteria class and Nitrospira family,were dominant in two samples,respectively.In sample TS,which was characterized by low pH,high sulfate,high iron,and high arsenide,two species（Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans）constituted 98.22% of the entire microbial community.Compared with sample TS,the microbial community in sample WK was more diversified according to the observation.Interestedly,the Legionella species,which was rarely observed in the low-pH environment,was detected in sample WK.This work helps us to further understand the diversity of microbial community living in extreme acid mine drainages with unique geochemistry and the tolerance capability of acidophiles to heavy metal.

关 键 词：microbial community acid mine drainage（AMD） tolerance capability PCR-based cloning approach 

分 类 号：X172[环境科学与工程—环境科学] X753