Aspirin and pravastatin reduce lectin-like oxidized low density lipoprotein receptor-1 expression, adhesion molecules and oxidative stress in human coronary artery endothelial cells  

作　　者：CHEN Jia-wei ZHOU Shi-bei TAN Zhi-ming 

机构地区：[1]Department of Anesthesiology, Fudan University Shanghai Cancer Center, Shanghai 200032, China

出　　处：《Chinese Medical Journal》2010年第12期1553-1560,共8页中华医学杂志（英文版）

摘　　要：Background Oxidative stress and inflammation are important steps in the pathogenesis of atherosclerosis. We postulated that therapeutic concentrations of aspirin and pravastatin, especially in combination, may suppress oxidative stress and inflammation in endothelial cells, and this concept was examined in human coronary artery endothelial cells （HCAECs）. Methods Human coronary artery endothelial cells were cultured and treated with oxidized-low density lipoprotein （ox-LDL, 60 ug/ml for 24 hours） alone, or pre-treated with aspirin （1, 2 or 5 mmol/L）, pravastatin （1, 5 or 10 umol/L） or their combination （1 mmol/L aspirin and 5umol/L pravastatin）, followed by ox-LDL treatment. After respective treatment, superoxide anion production, p38 mitogen activated protein kinase and transcription factor NF-KB activation, protein expression of lectin-like ox-LDL receptor-1 （LOX-1） and adhesion molecules, and monocyte adhesion were measured. Results Ox-LDL treatment greatly elicited its receptor LOX-1 expression, superoxide anion production and inflammatory response, which were minimally affected by low concentration of aspirin （1 mmol/L） or pravastatin （5umol/L）, but were markedly decreased by their combination. Activation of p38 mitogen activated protein kinase and NF-KB, the expression of intercellular adhesion molecule-1 and monocyte chemotactic protein-I, which were only mildly affected by aspirin or pravastatin alone, were significantly attenuated by their combination. As a consequence, monocyte adhesion to endothelial cells was markedly attenuated by the combination of the two agents. Well-known anti-oxidants a-tocopherol and y-tocopherol had similar inhibitory effects on ox-LDL-mediated oxidative stress and LOX-1 expression as well as monocyte adhesion as did the combination of aspirin and pravastatin. Conclusions These studies point to a positive interaction between aspirin and pravastatin with regard to endothelial bioloqy. Anti-oxidant and subsequent anti-inflammatory effect may be onBackground Oxidative stress and inflammation are important steps in the pathogenesis of atherosclerosis. We postulated that therapeutic concentrations of aspirin and pravastatin, especially in combination, may suppress oxidative stress and inflammation in endothelial cells, and this concept was examined in human coronary artery endothelial cells （HCAECs）. Methods Human coronary artery endothelial cells were cultured and treated with oxidized-low density lipoprotein （ox-LDL, 60 ug/ml for 24 hours） alone, or pre-treated with aspirin （1, 2 or 5 mmol/L）, pravastatin （1, 5 or 10 umol/L） or their combination （1 mmol/L aspirin and 5umol/L pravastatin）, followed by ox-LDL treatment. After respective treatment, superoxide anion production, p38 mitogen activated protein kinase and transcription factor NF-KB activation, protein expression of lectin-like ox-LDL receptor-1 （LOX-1） and adhesion molecules, and monocyte adhesion were measured. Results Ox-LDL treatment greatly elicited its receptor LOX-1 expression, superoxide anion production and inflammatory response, which were minimally affected by low concentration of aspirin （1 mmol/L） or pravastatin （5umol/L）, but were markedly decreased by their combination. Activation of p38 mitogen activated protein kinase and NF-KB, the expression of intercellular adhesion molecule-1 and monocyte chemotactic protein-I, which were only mildly affected by aspirin or pravastatin alone, were significantly attenuated by their combination. As a consequence, monocyte adhesion to endothelial cells was markedly attenuated by the combination of the two agents. Well-known anti-oxidants a-tocopherol and y-tocopherol had similar inhibitory effects on ox-LDL-mediated oxidative stress and LOX-1 expression as well as monocyte adhesion as did the combination of aspirin and pravastatin. Conclusions These studies point to a positive interaction between aspirin and pravastatin with regard to endothelial bioloqy. Anti-oxidant and subsequent anti-inflammatory effect may be on

关 键 词：ASPIRIN endothelial dysfunction HMG CoA reductase inhibitor INFLAMMATION oxidative stress 

分 类 号：R686[医药卫生—骨科学]