脑缺血再灌注后皮层微血管超微结构的实验研究  

Ultrastructural Characteristics of Cortical Microvasculature after Global Cerebral Ischemia-reperfusion

作　　者：马铁柱[1] 涂悦[1] 张赛[1] 孙世中[1] 

机构地区：[1]武警医学院附属医院脑系科中心,天津市300162

出　　处：《中国康复理论与实践》2010年第6期525-527,F0003,共4页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的研究急性脑缺血再灌注后皮层微血管超微结构的动态变化。方法将40只雄性大鼠随机分为假手术组(20只)和脑缺血再灌注组(20只)。采用线栓法制作大鼠大脑中动脉缺血模型,脑缺血1h后再灌注1、3、12、72h取材,复合甲基丙烯酸甲酯脑微血管铸型,制作脑微血管标本,扫描电镜观察正常大鼠脑皮层微血管和急性脑损伤后脑皮层微血管形态学的改变。结果与假手术组相比,脑缺血再灌注后出现皮质的血管受损迹象,随着时间推移,铸型血管可呈"豆芽"状甚至出现完全断裂产生"烛泪"状血管铸型断端,最后,进一步形成皮层无血管区。结论急性脑缺血再灌注后脑皮层微血管结构改变是导致脑微循环障碍的重要原因。Objective To investigate the dynamic changes in microvascular ultrastructure in the cortex after the acute cerebral ischemia-reperfusion.Methods 40 male rats were randomly divided into two groups（n=20 for sham operation group and cerebral ischemia-reperfusion group）.Cerebral ischemia-reperfusion model was produced using suture middle cerebral artery occlusion.Rats were sacrificed and the brain samples were adopted 1,3,12,72 h after ischemia-reperfusion,methyl methacrylate composite brain microvascular casting.The production of brain microvascular specimens,scanning electron microscopy of normal rat cerebral cortex microvessels and cerebral cortex of acute brain injury morphological changes in microvascular.Results Compared with the sham-operated group,cerebral ischemia-reperfusion in the cortex after the signs of vascular damage,then,vascular casting was to ＂bean＂ shape or even had a completely broken ＂tears candles＂ stump-like vascular casting,finally,to further the formation of a vascular zone cortex.Conclusion The structural changes of brain microvascular in the cortex after acute cerebral ischemia-reperfusion is an important cause of cerebral microcirculation in rats.

关 键 词：脑缺血再灌注 微血管 超微结构 

分 类 号：R743[医药卫生—神经病学与精神病学]