大孔树脂对凤尾草黄酮吸附分离特性研究的影响  

Effect of Macroporous Absorption Resin on Absorption and Separation of Pteris multifida Flavones

作　　者：黄运凤[1] 刘国凌[2] 

机构地区：[1]广州城市职业学院建筑环境与食品工程学院,广东广州510405 [2]韶关学院食品系,广东韶关512005

出　　处：《贵州农业科学》2010年第6期57-60,共4页Guizhou Agricultural Sciences

基　　金：广州市教育局科技攻关项目(62076)

摘　　要：选择5种大孔吸附树脂,比较其对凤尾草总黄酮的吸附率,筛选较优的凤尾草黄酮吸附剂,并探讨其吸附纯化工艺条件。结果表明,HPD-700树脂较适宜于凤尾草黄酮的提纯,且在树脂与样液比为1∶20(树脂质量与样液体积的数值比),pH 4.0,凤尾草粗提液浓度为1.513 mg/mL的条件下吸附,用70%的乙醇洗脱时能有较好的分离纯化效果。经纯化后,凤尾草黄酮纯度大于50%,符合中药有效部位研究要求。Five macroporous absorption resins were tested to absorb flavones from Pteris multifida to screen the optimum absorption agent and discuss its absorption and purification technology.The results showed that HPD-700 resin had the highest flavones absorption rate from crude Pteris multifida extract and the optimum absorption technology including 1∶20 of HPD-700 resin/ sample solution with1.513 mg/mL concentration and pH 4.0.The crude flavones was eluted with 70% ethanol and more than 50% purity of Pteris multifida flavones could be obtained,which meets research requirement of effective component of traditional Chinese medicine.

关 键 词：大孔树脂 凤尾草黄酮 吸附 解吸 

分 类 号：S567.9[农业科学—中草药栽培]