高铁饮食致C57BL/6小鼠黑质DA多巴胺能神经元选择性损伤的研究  

FERRIC DIET INDUCED SELECTIVE DEGENERATION OF DOPAMINERGIC NEURONS IN SUBSTANTIA NIGRA OF C57BL/6 MICE

作　　者：吕占云[1] 姜宏[1] 徐华敏[1] 谢俊霞[1] 

机构地区：[1]青岛大学国家生理学重点(培育)学科山东省神经相关疾病的机制与防治重点实验室,山东青岛266071

出　　处：《青岛大学医学院学报》2010年第4期298-300,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金(30870858);山东省自然科学杰出青年基金(JQ200807)资助项目

摘　　要：目的研究高铁饮食对C57BL/6小鼠黑质(SN)和中脑腹侧被盖区(VTA)多巴胺能神经元的不同损伤。方法将C57BL/6小鼠随机分为高铁饮食组和正常对照组,利用免疫组织化学染色法及铁染色技术检测两组小鼠SN和VTA的酪氨酸羟化酶(TH)阳性细胞数及铁水平的变化。结果高铁饮食组SN的TH阳性细胞数较正常对照组明显减少(t=16.31,P<0.001),铁染色阳性细胞数较对照组明显增高(t=13.01,P<0.001)。而两组小鼠VTA的TH阳性细胞数及铁染色阳性细胞数均无明显差别。结论铁在SN的选择性聚积参与了SN多巴胺能神经元的选择性损伤。Objective To investigate the effect of ferric diet on the degeneration of dopaminergic neurons between the substantia nigra（SN） and the ventral tegmental area（VTA） in C57BL/6 mice. Methods The male C57BL/6 mice were randomly divided into two groups： ferric-diet group and normal-diet group.Tyrosine hydroxylase（TH） positive cells and iron positive cells in SN and VTA were determined using immunohistochemistry and iron staining. Results Compared with those of the control group,the number of TH-positive cells in SN was significantly decreased（t=16.31,P0.001） and the number of iron-positive cells significantly increased（t=13.01,P0.001） in ferric-diet group.The numbers of both TH-positive cells and iron-positive cells were not significantly different in the VTA between the two groups. Conclusion Selective iron accumulation in SN is involved in the selective degeneration of dopamine neurons in SN.

关 键 词：铁 黑质 腹侧被盖区 神经元 创伤和损伤 酪氨酸单氧化酶 小鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]