结肠癌RKO细胞P物质和NK-1免疫细胞化学检测  

IMMUNOCYTOCHEMICAL EXPRESSIONS OF SUBSTANCE P AND NEUROKININ-1 IN RKO CELLS OF COLON CANCER

作　　者：赵岭岭[1] 陈桦[1] 王继刚[2] 

机构地区：[1]青岛大学医学院附属青岛市市立医院,山东青岛266071 [2]青岛大学医学院附属医院病理科

出　　处：《齐鲁医学杂志》2010年第4期289-290,293,共3页Medical Journal of Qilu

基　　金：青岛市科技发展计划基金资助项目(08-2-1-4-nsh)

摘　　要：目的探讨P物质及其受体神经激肽1(NK-1)在体外培养的结肠癌细胞系RKO中的表达及免疫细胞化学定位。方法将RKO细胞接种于提前放入盖玻片的6孔板进行细胞培养,细胞融合30%时将盖玻片取出进行免疫细胞化学染色。结果在RKO细胞系中P物质和NK-1呈阳性表达,P物质阳性表达多数位于细胞浆;而NK-1阳性表达多数位于细胞浆,少数位于细胞膜。结论神经内分泌机制可能参与了结肠癌的发病过程。Objective To investigate the immuno-expressions of substance P and NK-1 in RKO cell lines of colon cancer.Methods RKO cells were inoculated on the cover glass and cultured on 6-well plate.The cover glass was removed from the plate upon 30% of cell fusion,and the expressions of SP and NK-1 of the cultured cells were checked via immunocytochemical staining.Results Positive expressions of substance P and NK-1 were mainly found within the cytoplasm of the RKO cells,while a few of NK-1 noted on the membrane. Conclusion The neuroendocrine mechanism might involve in the process of morbidity of colon cancer.

关 键 词：结肠肿瘤 RKO细胞系 P物质 受体 神经激肽 免疫细胞化学 

分 类 号：R737.9[医药卫生—肿瘤]