采后硅酸钠处理及损伤接种对厚皮甜瓜CHS基因的诱导表达  

Induced expression of CHS gene in muskmelon fruit treated with sodium silicate and challenged with Trichothecium roseum

作　　者：高雄杰[1,2] 赵继荣[1,2] 毕阳[1] 李科玮[1] 李红霞[1] 伍利芬[3] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [3]甘肃农业大学农学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2010年第3期48-51,共4页Journal of Gansu Agricultural University

基　　金：国家自然科学基金资助项目(30671465)

摘　　要：以厚皮甜瓜品种"银帝"为试材,根据黄瓜查儿酮合成酶(CHS)基因设计引物,运用半定量RT-PCR技术,研究了采后100mmol·L-1硅酸钠浸泡处理对甜瓜果实CHS基因表达的影响.结果表明:PCR扩增所得片段(494bp)与黄瓜CHS基因同源,相似性为94%,可用于半定量RT-PCR分析;CHS基因在处理和对照中的表达丰度极低,无法检测;损伤接种T.roseum后第4d,CHS基因大量表达,且硅酸钠处理+损伤接种与对照+损伤接种差异达到最大,前者高出后者12%.The primer used in this experiment was designed according to the CHS gene from cucumber.Semi quantitative RT-PCR was used for evaluating the effect of sodium silicate dipping at 100 mmol·L^-1 on CHS gene expression of muskmelon fruit （Cucumis melon L. cv. Yindi）. The results showed that the PCR segments（494 bp）which shared 94 % homology with the cucumber CHS gene could be used for RT-PCR;the expression of CHS gene in the treated fruit and the control were too low to detect.However,an abundance expression of CHS gene was found 4 days after challenged with T.roseum,and 12 % higher in the treated fruit ＋ challenged than control ＋ challenged.

关 键 词：厚皮甜瓜 硅酸钠 CHS基因 诱导表达 

分 类 号：S652[农业科学—果树学]