斑点叉尾鮰气单胞菌的分离与鉴定  

Isolation and Identification of Aeromonas from Channel Catfish (Ictalurus punctatus)

作　　者：李艳和[1] 杨克礼[2] 佘磊[1] 徐涤平[2] 王永杰[1] 

机构地区：[1]安徽省农业科学院水产研究所,安徽合肥230031 [2]湖北省农业科学院畜牧兽医研究所,动物胚胎工程及分子育种湖北省重点实验室

出　　处：《青岛农业大学学报（自然科学版）》2010年第2期150-153,158,共5页Journal of Qingdao Agricultural University(Natural Science)

基　　金：安徽省科技攻关计划项目(08010302171);安徽省115创新团队资助

摘　　要：集约化养殖中,气单胞菌属细菌已成为危害渔业生产的重要致病菌。本研究自患病斑点叉尾鮰体内分离出5株气单胞菌,经形态学观察、生长特性及生理生化反应测试、鉴定,其中AHIA02、AHIA04、AHIA05为嗜水气单胞菌;AHIA01、AHIA03为豚鼠气单胞菌。用20种常规抗生素分别对分离菌株进行药物敏感性试验,结果表明:所有分离菌株仅对奥复星、复方新诺明、先锋必素等3种抗生素敏感,交叉耐药性和多重耐药性现象严重。The bacteria Aeromonas have been the important pathogenic bacteria which endanger the fishery production in the intensive aquatic culture. In this study,five strains of bacteria were isolated from the tissues of diseased channel catfish,Ictalurus punctatus. Bacterial morphology,cultural characteristics,and biochemical identification were carried out. According to the morphological feature and biochemical characteristics of the isolated strains, Strain AHIA02,AHIA04 and AHIA05 belong to Aeromonas hydrophila,and strain AHIA01 and AHIA03 belong to Aeromonas caviae. The results of drug sensitivity test showed that all the isolated strains have serious multidrug resistance and cross resistance,and only sensitive to Ofloxacin,chemitrim and Cefoperazone.

关 键 词：气单胞菌 斑点叉尾鮰 分离鉴定 药敏试验 

分 类 号：S917.1[农业科学—水产科学]