柚皮提取物对植物病原真菌的抑菌活性测定  

Study on the Antifungal Activity of Extracts from Shaddock Peels

作　　者：肖琳[1] 金玉兰[1] 

机构地区：[1]青岛农业大学化学与药学院,山东青岛266109

出　　处：《青岛农业大学学报（自然科学版）》2010年第2期172-175,共4页Journal of Qingdao Agricultural University(Natural Science)

摘　　要：通过测定柚皮的乙醇提取物以及各萃取部分对植物病原真菌的抑菌活性,寻找柚皮中的农用活性物质,为其综合开发利用提供科学依据。采用菌丝生长速率法测定了柚皮乙醇提取物对8种植物病原菌的抑制作用。在供试浓度为5.0mg/ml时,柚皮乙醇提取物对苹果腐烂病菌(Valsa mali)、辣椒炭疽菌(Colletotrichum capsici)和棉花枯萎菌(Fusarium oxysporum vasinfectum)的抑菌活性较高,抑菌率分别为80.7%、72.9%、62.5%。柚皮乙醇提取物的乙酸乙酯层的抑菌活性较高,乙酸乙酯层对苹果腐烂病菌的抑菌作用最好,EC50达0.45mg/ml,柚皮乙醇提取物的乙酸乙酯层是进一步研究的重点。The antifungal activity of ethanolic extract and fractions were determined from shaddock peels. The aim was to develop and utilize comprehensively agricultural substances of shaddock peels. Using growth rate methods, antifungal activity of shaddock peels was researched in this experiment. The results showed that the shaddock peels ethanolic extract was more effective against Valsa mali,Colletotrichum capsici and Gibberella zeae. The inhibition rates were 80. 7% ,72. 9% ,59. 8% at 5. 0mg/ml,respectively. The EC50 of ethyl acetate partition was 0. 45 mg/ml against V. mali. The ethyl acetate fraction was the most active partition and could be acted as the emphases for further study.

关 键 词：柚皮 植物病原真菌 抑菌活性 

分 类 号：S482.292[农业科学—农药学]