响应面优化法在纤维素酶合成培养基设计上的应用  

Statistical Optimization of Process Parameters on Cellulase Production

作　　者：张晓萍[1] 杨静[1] 勇强[1] 余世袁[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京210037

出　　处：《林产化学与工业》2010年第3期29-34,共6页Chemistry and Industry of Forest Products

基　　金：国家863计划资助(2008AA05Z401);江苏高校自然科学重大基础研究项目(06KJA22015)

摘　　要：以里氏木霉(Trichoderma reesei)RUTC30为产酶菌株,经酵母发酵除去葡萄糖后的脱葡萄糖淀粉水解液为碳源,采用Plackett-Burman(PB)设计法寻找培养基组成中对产酶影响最大的因素,并经响应面试验设计优化最佳产酶条件。试验得到对产酶影响最大的两个因素分别为脱葡萄糖淀粉水解液质量浓度和氯化钙质量浓度,经最陡爬坡和响应面试验建立了滤纸酶活与两者之间的模型。对此模型求解得到,当脱葡萄糖淀粉水解液质量浓度为28.85 g/L、氯化钙质量浓度为0.67 g/L时,产酶120 h理论酶活为7.52 FPIU/mL,156 h达到最大酶活为11.16 FPIU/mL,β-葡萄糖苷酶活最大为0.79 IU/mL。Placket-Burman（PB） design was applied to discover the most significant parameters affecting cellulase production by Trichoderma reesei RUT C30 on deglucosed starch obtained from yeast fermentation,and the condition of cellulase production was optimized by response surface method（RSM）.Results showed that mass concentration of hydrolysate from hydrolysis of deglucosed starch and mass concentration of CaO were the two most significant parameters.The model between enzyme activity and these two parameters was build through the path of steepest ascent and RSM.Under optimized conditions,the levels of these two parameters were respectively 28.85 and 0.67g/L.The theoretical enzyme activity from 120 h fermentation was 7.52FPIU/mL,and the maximal value was 11.16FPIU/mL from 156h fermentation.The maximal glucosidase activity reached 0.79 IU/mL.

关 键 词：脱葡萄糖淀粉水解液 纤维素酶 响应面法 

分 类 号：TQ351.0[化学工程] Q55[生物学—生物化学]