油茶14-3-3蛋白基因的cDNA克隆及序列分析  

Cloning and Sequence Analysis of a 14-3-3 Protein Gene from Camellia oleifera

作　　者：刘巧[1] 谭晓风[1,2,3] 胡孝义[1] 田晓明[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004 [2]中南林业科技大学林学院,湖南长沙410004 [3]国家油茶科学中心生物技术实验室,湖南长沙410004

出　　处：《江西农业大学学报》2010年第3期541-546,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家"十一五"科技支撑项目(2006BAD18B0204;2006BAD01A1706);国家自然科学基金(30371184);湖南省科学技术厅科技计划项目(06FJ4111)

摘　　要：以构建的油茶近成熟种子cDNA文库和EST文库为基础,采用分子克隆技术,分离克隆1个14-3-3蛋白的全长cDNA序列,由1 156个核苷酸组成,5’非编码区57 bp,3’非编码区301 bp,开放阅读框长777 bp,编码259个氨基酸。该基因编码蛋白的分子质量为29.466 ku,等电点4.78,无信号肽序列,是非分泌蛋白,命名为Co-14-3-3a。推测的二级结构有9个α-螺旋,1个反平行的β-折叠,位于αC和αD之间。A cDNA library and an EST library of Camellia oleifera were constructed.A 14-3-3 protein gene was cloned from Camellia oleifera.This gene is 1 156 bp in length,including 57 bp of 5＇untranslated region and 301 bp of 3＇untranslated region.It has an open reading flame of 777 bp,encoding a protein of 259 amino acid residues with protein molecular weight of 29.466 ku and isoelectric point of 4.78.No signal peptide sequence was found,which shows it＇s a non-secreted protein.This gene is named Co-14-3-3a.The speculated secondary structure of Co-14-3-3a consists of nine helices and an extended-beta located between αC and αD.

关 键 词：油茶 14-3-3蛋白 基因克隆 序列分析 结构预测 

分 类 号：R513.3[医药卫生—内科学] S794.4[医药卫生—临床医学]