血清IL-10水平及其基因-1082A/G位点单核苷酸多态性与胃窦癌恶病质的关系  

ASSOCIATION OF IL-10 LEVELS AND-1082A/G MONONUCLEOTIDE POLYMORPHISM WITH CACHEXIA IN PATIENTS WITH CANCER OF GASTRIC ANTRUM

作　　者：孙风波[1] 张佃良[1] 郑红梅[1] 宋波 

机构地区：[1]青岛大学医学院附属医院普通外科,山东青岛266003 [2]烟台山医院急诊科

出　　处：《齐鲁医学杂志》2010年第3期189-191,194,共4页Medical Journal of Qilu

基　　金：山东省自然科学基金资助项目(Y2006C20);山东省科学技术发展计划资助项目(2008GG30002034)

摘　　要：目的探讨胃窦癌病人血清细胞因子白细胞介素10（IL-10）水平及-1082A/G位点单核苷酸多态性与恶病质发生的关系。方法采用放射免疫学方法检测150例胃窦癌病人及135例健康人（对照组）血清IL-10水平。用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测胃窦癌病人IL-10基因-1082A/G位点单核苷酸多态性。结果胃窦癌病人血清IL-10水平较对照组显著升高（Z=-11.862,P〈0.001）,胃窦癌Ⅲ、Ⅳ期病人血清IL-10水平较Ⅰ、Ⅱ期显著升高（Z=-10.028,P〈0.001）。胃窦癌恶病质病人血清IL-10水平较非恶病质病人显著升高（Z=-10.369,P〈0.001）。Logistic回归分析显示,IL-10为恶病质发生的高风险性因素（OR=1.559,95%CI=1.299-1.870,P〈0.001）。单核苷酸多态性分析显示,胃窦癌恶病质病人IL-10基因-1082G等位基因频率较非恶病质病人显著升高（χ2=3.953,P〈0.05）。胃窦癌恶病质病人IL-10基因-1082AG基因型频率较非恶病质病人显著升高（χ2=4.511,P〈0.05）。Logistic回归分析显示,校正肿瘤分期后,IL-10基因-1082AG基因型为胃窦癌恶病质的高风险性因素（OR=2.295,95%CI=1.029-5.117,P〈0.05）。结论血清IL-10水平高及IL-10基因-1082AG基因型与胃窦癌病人恶病质的发生具有相关性。Objective To explore the association of serum level of interleukin-10（IL-10） and-1082 A/G mononucleotide polymorphism（SNP） in patients with cachexia caused by cancer of gastric antrum. Methods Radioimmunoassay was used toexamine the serum level of IL-10 in 150 cancer patients and 135 healthy controls.The SNP of IL-10 gene-1082G/A was analyzedusing polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. Results The serum IL-10 was significantly higher in the patients than in controls（Z=-11.862,P0.001）.The serum IL-10 in patients with stageⅢ/Ⅳ cancer was significantly higher than that in patients with stageⅠ/Ⅱ（Z=-10.028,P0.001）.The serum IL-10 in cachectic patients was significantly higher than that in non-cachectic patients（Z=-10.369,P0.001）.Logistic regression analysis showed that IL-10 was associated with an odds ratio of 1.599（95%CI=1.299-1.870,P0.001） for cachexia.SNP analysis showed that a significantly increased frequency of-1082G allele was noted in patients with cachexia compared with patients without cachexia（χ2=3.953,P0.05）.The frequency of-1082AG genotype was significantly elevated in the cachectic than those without（χ2=4.511,P0.05）.In a logistic regression analysis adjusted for carcinoma staging,the-1082AG genotype was associated with an odds ratio of 2.295（95% CI=1.029-5.117,P0.05） for cachexia. Conclusion High serum levels of IL-10 and-1082AG genotype are associated with the occurrence of cachexia in patients with cancer of gastric antrum.

关 键 词：白细胞介素10 多态性 单核苷酸 胃肿瘤 恶病质 

分 类 号：R735.2[医药卫生—肿瘤]