酪氨酸-钐(Ⅲ)配合物与鲱鱼精DNA的作用方式  

Binding mode between Tyr-Sm(Ⅲ) complex and herring sperm DNA

作　　者：赵娜[1] 王兴明[1] 张艳[1] 胡亚敏[1] 丁立生[2] 

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010 [2]中国科学院成都生物研究所,四川成都610041

出　　处：《中南大学学报（自然科学版）》2010年第3期876-882,共7页Journal of Central South University:Science and Technology

基　　金：国家自然科学基金资助项目(30572254);西南科技大学研究生创新基金资助项目(09ycjj14)

摘　　要：采用UV光谱法、荧光光谱法,在pH=7.40的生理环境中用摩尔比法确定了Sm(Ⅲ)与Tyr(酪氨酸)结合的物质的量比n(Sm(Ⅲ)):n(Tyr)=1:3,Sm(Ⅲ)(Tyr)3配合物与hs(鲱鱼精)DNA结合的物质的量比n(Sm(Ⅲ)(Tyr)3):n(DNA)=3:1。用双倒数法确定了结合常数K2Θ98.15K=9.97×104L/mol和K3Θ10.15K=7.56×103L/mol。化学热力学研究显示配合物Sm(Ⅲ)(Tyr)3与hsDNA的结合过程为焓驱动;结合Scatchard法和黏度法,确定了配合物Sm(Ⅲ)(Tyr)3与hsDNA之间的作用方式为沟渠作用和嵌插作用。With the methods of UV spectra,fluorescence spectra in physiological pH environment （pH=7.40）,the binding ratio of n（Sm（ Ⅲ） ）：n（Tyr）=1：3 and n（Sm（Ⅲ ）（Tyr）3）：n（DNA）=3：1 were confirmed by molar ratio method. The binding constants of K 2Θ9 8.15 K=9.97×104 L/mol and K 3Θ1 0.15 K =7.56×103 L/mol were confirmed by double reciprocal method. Thermodynamic function computation demonstrates that △r HmΘ is the primary driving power of the interaction of Sm（Ⅲ ）（Tyr）3 and DNA. Combined with Scatchard method and viscosity method,the results suggest that the interaction mode between Sm（Ⅲ ）（Tyr）3 complex and herring sperm DNA are groove binding and intercalation binding.

关 键 词：酪氨酸 钐(Ⅲ) 鲱鱼精DNA 配合物 作用方式 

分 类 号：O629.74[理学—有机化学]