低温选择大黄鱼(Pseudosciaena crocea)的肝脏蛋白质组双向电泳分析  

2-DE ANALYSIS OF LIVER OF PSEUDOSCIAENA CROCEA AFTER LOW TEMPERATURE BREEDING

作　　者：李明云[1] 吴玉珍[1] 冀德伟[1] 吴海庆[1] 陈炯[1] 史雨红[1] 罗海忠 柳敏海 傅荣兵 

机构地区：[1]应用海洋生物技术教育部重点实验室宁波大学,宁波315211 [2]浙江省舟山市水产研究所,舟山316000

出　　处：《海洋与湖沼》2010年第3期348-351,共4页Oceanologia Et Limnologia Sinica

基　　金：国家高技术研究发展计划(863)项目,2006AA10A405号;浙江省重大项目,2006C12005号;宁波市重大项目,2008C10022号;长江学者和创新团队发展计划项目,IRT0734号

摘　　要：采用双向电泳技术,对低温选择组与对照组大黄鱼进行肝脏蛋白质组双向电泳分析。结果表明,低温选择组肝脏蛋白点1673±47个,对照组1651±43个,共有19个蛋白点经低温选择后,表达量显著变化。从中挑选3个差异蛋白点进行肽指纹图谱(MALDI-TOF-MS)或串联质谱(LC-MS-MS)分析,然后MS-BLAST数据库搜寻。低温选择组大黄鱼肝脏MHC和CFL2蛋白表达显著上调,而2-CysPrxs蛋白表达显著下调。说明低温选育组大黄鱼机体抗细胞凋亡能力明显增强,预示低温选择后留下的大黄鱼在低温环境中生存能力更强。We used 2-DE to compare the liver proteomes of Pseudosciaena crocea with or without low-temperature breeding,and found similar number of proteins expressed in the low-temperature breeding group （1673±47） and the control group （1651±43）.The expressions of 19 proteins were significantly altered after low temperature breeding.Three proteins （MHC,CFL2 and 2-Cys Prxs） were identified by MALDI-TOF-MS/LC-MS-MS and MS-BLAST searching.The expressions of MHC and CFL2 protein were up-regulated remarkably,whereas the expression of 2-Cys Prxs was down-regulated remarkably,suggesting the anti-apoptosis ability of the low-temperature breeding group is strengthed.

关 键 词：大黄鱼 低温选育 肝脏 双向电泳 

分 类 号：S965.322[农业科学—水产养殖] Q523[农业科学—水产科学]