白藜芦醇对LPS刺激下NF-κB核转位影响  

EFFECT OF RESVERATRO1 ON TRANSLOCATION OF NF-κB INTO NUCLEAR INDUCED BY LPS

作　　者：姜鲜[1] 李佳[2] 匡红英[1] 廖常丽[1] 欧册华[1] 

机构地区：[1]四川泸州医学院第一附属医院麻醉科,泸州646000 [2]泸州医学院第一附属医院内分泌科,泸州646000

出　　处：《现代预防医学》2010年第14期2706-2707,共2页Modern Preventive Medicine

基　　金：四川省卫生厅课题(080171)

摘　　要：[目的]观察白藜芦醇对脂多糖刺激小鼠RAW264.7细胞的NF-κB核转位影响。[方法]体外培养小鼠RAW264.7细胞,脂多糖刺激小鼠RAW264.7细胞,用不同浓度白藜芦醇进行干预,免疫荧光法结合激光共聚焦显微镜检测NF-κB核转位情况。[结果]共聚焦显微镜下观察到正常组细胞NF-κB荧光标记主要分布于胞浆区,核区很少,LPS刺激后NF-κB荧光标记浓集于核区,胞浆区少见;白藜芦醇预处理后再给予LPS刺激的荧光标记主要位于胞浆区,以100、50μmol.L-1组较明显。[结论]白藜芦醇能抑制脂多糖刺激下小鼠RAW264.7细胞的NF-κB核转位。[Objective] To observe the effects of Resveratro1 on translocation of NF-κB into nuclear in RAW 264.7 cells induced by LPS.[Methods] RAW264.7 cell lines were cultivated and induced by LPS.The nuclear translocation of NF-κB in RAW264.7 cells were detected by immunofluorescence and laser scanning confocal microscope.[Results] The Cy3-labeled NF-κB was mainly located in the nuclei of RAW264.7 cells induced by LPS.The Cy3-labeled NF-κB was mainly located in the cytoplasma of RAW264.7 cells pretreated with resveratro1（100 and 50μmol.L^-1 groups were obvious）.[Conclusion] Resveratro1 could inhibit the nuclear translocation of NF-κB in RAW264.7 cells induced by LPS.

关 键 词：白藜芦醇 脂多糖 核因子-ΚB 

分 类 号：R363[医药卫生—病理学]