磺化壳聚糖对MCF-7的体外抑制作用研究  

Inhibiton of Sulfonated Chitosan on Human Breast Cancer Cell Line MCF-7

作　　者：张爱英[1] 王学东[2] 邓树娥[2] 孙凤祥[2] 王振松[3] 

机构地区：[1]潍坊医学院免疫学教研室山东省高校免疫学重点实验室,山东潍坊261053 [2]潍坊医学院化学教研室,山东潍坊261042 [3]山东中医药大学第二附属医院放射科,山东济南250001

出　　处：《现代生物医学进展》2010年第10期1842-1844,1853,共4页Progress in Modern Biomedicine

摘　　要：目的:研究磺化壳聚糖(SCTS)对体外培养的人乳腺癌细胞的增殖抑制和凋亡的作用。方法:用不同浓度磺化壳聚糖对体外培养人乳腺癌细胞MCF-7进行干预,MTT法检测SCTS对MCF-7细胞增殖的抑制作用;显微荧光法、流式细胞术检测细胞凋亡。结果:磺化壳聚糖抑制MCF-7细胞增殖,且呈时间、剂量依赖性;镜下可见凋亡细胞的形态学改变、FCM显示G0/G1期细胞增加,而S期细胞减少。结论:磺化壳聚糖可有效抑制人乳腺癌细胞MCF-7增殖,促进细胞凋亡。Objective：To study the effect of sulfonated chitosan（SCTS） on proliferation,apoptosis of MCF-7 cells in vitro.Methods：MCF-7 human breast carcinoma cell lines were treated with SCTS in vitro,cell proliferation was evaluated by MTT assay;cell apoptosis was evaluated by microfluormetric and FCM.Results：sulfonated chitosan can inhibit the growth of human breast cells MCF-7 were efficiently inhibited,in dose-dependent and time-dependent;The morphological changes of apoptosis were found under microfluormetric;FCM indicated the increased cell population in the G0/G1 phase was associated with a decreased cell number in the S phase.Conclusion：Sulfonated chitosan can effectively inhibit proliferation and induce the apoptosis of human breast carcinom cells MCF-7 in vitro.

关 键 词：磺化壳聚糖 MCF-7细胞 抗肿瘤 凋亡 

分 类 号：R730.231[医药卫生—肿瘤]