多巴胺D4受体基因启动子区功能多态性与海洛因依赖的相关性研究  

Association between the Polymorphism in the Promoter Region of Dopamine D4 Receptor Gene and Heroin Dependence

作　　者：贾伟[1] 师建国[1] 敖磊[1] 张蕊[1] 

机构地区：[1]西安市精神卫生中心,陕西西安710065

出　　处：《现代生物医学进展》2010年第11期2061-2063,共3页Progress in Modern Biomedicine

基　　金：西安地区汉族人群阿片受体基因多态性与美沙酮维持治疗的关联研究(SF08011);陕西省科学技术研究发展计划项目(2008K15-07)

摘　　要：目的:探讨多巴胺D4受体(DRD4)基因启动子区的3个功能多态性位点与海洛因依赖的相关性。方法:严格按照诊断标准,选取无亲缘关系的海洛因依赖患者212例及健康对照组200例提取基因组DNA,采用聚合酶链反应及等位基因特异性扩增技术检测DRD4基因启动子区-521C/T、-616C/G、及-1240L/S3个功能多态性位点的基因型,采用HaploView及SPSS11.5软件分析各位点基因型、等位基因频率及组间差异。结果:DRD4基因-521C/T的基因型及等位基因频率分布在海洛因依赖组与正常对照组存在显著性差异(p<0.05)。结论:多巴胺D4受体(DRD4)基因-521C/T多态性与海洛因依赖相关联,T等位基因可能是海洛因依赖的风险因子。Objective：To study a possible association between the three functional polymorphisms in the promoter region of dopamine D4 receptor （DRD4） gene and heroin dependence.Methods：Genomic DNA was isolated from the venous blood leukocytes of 212 unrelated patients with heroin dependence and 200 healthy unrelated individuals （Control group）.Polymorphism of DRD4,-521C/T,-616C/G, and-1240L/S, were genotyped by the allele-specific primer （ASP） PCR.Genotype, and allele frequencies were analysed by HaploView and SPSS11.5 software.Results：There were significant differences in both allele and genotype frequencies of DRD4-521C/T between the Study and Control group.Conclusions：There is an association between the DRD4-521C/T polymorphism and heroin dependence.The individuals with T allele are susceptible to heroin dependence.

关 键 词：多巴胺D4受体启动子 海洛因依赖 多态性 

分 类 号：R-3[医药卫生]