银杏内酯B对胆固醇和apoE4共处理海马神经元APP的影响  

Effects of Ginkgolide B on the β-amyloid precursor protein of hippocampi neurons exposed to cholesterol and apolipoprotein E4

作　　者：马轶文[1] 范英昌[1] 姜希娟[1] 郭茂娟[2] 卢斌[2] 

机构地区：[1]天津中医药大学病理学教研室,300192 [2]天津中医药大学实验教学部,300192

出　　处：《临床与实验病理学杂志》2010年第3期345-347,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：天津市自然科学基金(No08JCYBJC10800);天津市卫生局基金(No09KZ115)

摘　　要：目的探讨银杏内酯B对高胆固醇和apoE4共损伤海马神经元APP基因和蛋白水平的影响。方法应用高胆固醇和apoE4共处理海马神经元,形成高胆固醇细胞损伤模型。采用实时定量RTPCR的方法检测APP mRNA的表达,采用Western-blot测定APP蛋白水平。结果高胆固醇可以上调APP mRNA的表达,提高APP蛋白水平;银杏内酯B下调APP mRNA和蛋白水平的基因表达。结论高胆固醇可以通过促进β-淀粉样蛋白形成,参与阿尔茨海默病(Alzheimer’s disease,AD)发生、发展,银杏内酯B可以有效抑制β-淀粉样蛋白形成,为AD的治疗提供了一个可行途径。Purpose To observe the effect of Ginkgolide B on the APP of cultured hippocampi neurons exposed to cholesterol and apoliptein E4.Methods In order to get cellular damage model,use high concentration cholesterol and apoliptein E4 to deal with the hippocampi neurons.RT-PCR was applied to detect the expression of APP mRNA and Western-blot was used to assay the APP protein level.Result cholesterol can up-regulate APP mRNA and raise the APP protein level;Ginkgolide B down-regulate APP mRNA and protein gene expression.Conclusion High concentration cholesterol promote the formation of β-amyloid,therefore,take part in the progress of Alzheimer′s disease（AD）,Ginkgolide B could effect on inhibit β-amyloid protein,and it may be a feasible way to treat AD.

关 键 词：阿尔茨海默病 胆固醇 载脂蛋白E4 银杏内酯B β-淀粉样蛋白前体蛋白 Β-淀粉样蛋白 

分 类 号：R745.7[医药卫生—神经病学与精神病学]