虾青素对对乙酰氨基酚所致小鼠肝损伤的保护作用  

Protective effect of astaxanthin on paracetamol-induced hepatic damage in mice

作　　者：张真[1] 扶艳[1] 梁信芳[1] 苏美琴[1] 张贤华[1] 朱铉[1] 

机构地区：[1]厦门大学医学院药学系,福建厦门361005

出　　处：《中国新药与临床杂志》2010年第6期433-436,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：福建省厦门市科技计划项目(3502Z20093012)

摘　　要：目的研究虾青素对对乙酰氨基酚所致小鼠肝损伤的保护作用及其作用机制。方法 40只小鼠随机分为5组(n=8):正常对照组、模型组和虾青素低、中、高剂量(50、125和250 mg.kg-1)组。各剂量组小鼠给予虾青素连续灌胃7 d后一次性腹腔注射对乙酰氨基酚400 mg.kg-1制备急性肝损伤模型,光镜观察肝脏的组织学改变,测定血清和肝组织的生化指标。结果与模型组比较,虾青素组血清中丙氨酸转氨酶、天冬氨酸转氨酶含量明显降低(P<0.01),肝组织中丙二醛含量降低,超氧化物歧化酶、谷胱甘肽过氧化物酶活性提高(P<0.01)。结论虾青素能增强小鼠体内酶防御系统功能,提高清除自由基的能力,对对乙酰氨基酚所致小鼠肝损伤具有明显保护作用。AIM To study the protective effect of astaxanthin on paracetamol-indueed hepatic damage in mice. METHODS Forty mice were randomly divided into normal control group, model group, astaxanthin low, medium and high-dose （50, 125, and 250 mg·kg-1） groups, with eight mice in each group. Mice in astaxanthin low, medium and high-dose groups were administered with astaxanthin for 7 d consecutively. After 7 days, all the mice were then intraperitoneally given paracetamol at a dose of 400 mg-kg-1. The levels of ALT, AST in serum and MDA, SOD, GSH-PX in hepatic tissue were measured. RESULTS Compared with the model group, in the astaxanthin groups, the levels of ALT, AST in serum were decreased significantly （P 〈 0.01）, the level of MDA was decreased while activities of SOD and GSH-PX were increased in hepatic tissue （P 〈 0.01）. CONCLUSION Astaxanthin has good protective effect on paraeetamol-induced hepatic damage in mice. Eliminating free radical, repairing damaged cell membrane and enhancing defense enzyme activities in mice may be the mechanism of action of astaxanthin.

关 键 词：对乙酰氨基酚 肝炎 中毒性 模型 动物 小鼠 肝损伤 虾青素 

分 类 号：R965[医药卫生—药理学]