溶剂、酰化剂和底物浓度对手性氯醇酶促酯化的影响  

Effects of Organic Solvent,Acylating Agent and Substrate Concentration on Lipase catalyzed Esterification of Chiral Chlorohydrin

作　　者：庄英萍[1] 许建和[1] 周利[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室

出　　处：《华东理工大学学报（自然科学版）》1999年第1期43-46,共4页Journal of East China University of Science and Technology

基　　金：国家自然科学基金资助项目(29506043);上海市科委基金资助

摘　　要：利用脂肪酶在非水相体系中催化的对映选择性酯化反应，对外消旋手性药物中间体（±）－１－氯－３－（１－萘氧）－２－丙醇（简称萘氧氯丙醇）进行了拆分，考察了溶剂、酰化剂和底物浓度对酶活性和选择性的影响。结果表明，当溶剂为异辛烷，酰化剂为醋酸酐、底物浓度为氯醇１００ｍｍｏｌ／Ｌ、酸酐５０ｍｍｏｌ／Ｌ时，酶显示出最佳的催化活性和选择性，产物酯的光学纯度可达９０％。Efficient kinetic resolution of a racemic chlorohydrin, (±) 1 chloro 3 (1 naphthyloxy) 2 propanol, was achieved by lipase catalyzed enantioselective esterification in non aqueous media. The best solvent is isooctane and the best acylating agent is acetic anhydride with respect to either the catalytic activity or the enantioselectivity of the enzyme. The optimum concentrations of substrates were shown to be chlorohydrim 100 mmol/L and anhydride 50 mmol/L respecively.

关 键 词：(±)-1-氯-3-(1-萘氧)-2-丙醇 光学拆分 酶促酯化 脂肪酶 

分 类 号：O623.624.1[理学—有机化学] O621.256.4[理学—化学]