陆地棉突变体腺体形成时期的基因表达谱  

Gene Expression Profiling during Morphogenesis of Gland in Upland Cotton Mutant

作　　者：陈敏[1,2] 江明锋[1] 蔡应繁[2] 孙全[2] 何晓红[2] 江怀仲[2] 谢永芳[2] 莫剑川[2] 高运玲[2] 潘正[2] 陈珺[2] 伍婷玉[2] 王玲[2] 朱小燕[2] 张锐[2] 袁友禄[3] 石玉真[3] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]重庆邮电大学生物信息学院,重庆400065 [3]中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,河南安阳455000

出　　处：《江苏农业学报》2010年第3期476-481,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(30771311、30440032);重庆自然科学基金项目(2007BB1328、BB1329)

摘　　要：为了解棉花腺体形成时期相关基因的表达谱,分别抽提陆地棉湘棉-18和N5在腺体形成时期的RNA,采用Affymetrix芯片,扫描采集数据后进行分析。结果显示,在腺体形成时期有明显差异表达的基因共有514条,包括209条上调基因和305条下调基因;在514条差异表达基因中有275条(53.5%)与GenBank中已知的基因具有较高的相似性,这些基因主要涉及防御反应及异戊二烯生物合成等,有239条(46.5%)在GenBank中无任何注释。KOBAS分析结果显示,这些差异基因在KEGG数据库上涉及19条生化途径。说明,腺体形成过程是一个复杂的基因网络调控过程,涉及多条萜类化合物生物合成途径及其他与防御基因相关的途径。To identify gene expression patterns of pigment gland morphogenesis in cotton,genes involved in pigment gland morphogenesis in upland cotton varieties Xiang-18 and N5 were profiled using Affymetrix cotton gene chip.The results showed that there were 514 genes expressed differentially during gland morphogenesis,of which 209 genes were up-regulated and 305 genes were down-regulated.Out of the 514 genes,275（53.5%） genes shared high similarity in nucleic sequence with known genes in GenBank,and the remained 239（46.5%） genes were unknown.The known genes were mainly involved in defense response and isoprenoid biosynthetic process.The KOBAS（KEGG Orthology-Based Annotation System） analysis indicated that the differentially expressed genes were involved in 19 metabolic pathways.The results suggested that pigment gland morphogenesis in cotton was regulated by a complicate gene network,including several terpenoid biosynthetic pathways and plant defense-related pathways.

关 键 词：棉花 棉酚 腺体形成 基因芯片 表达谱 

分 类 号：S562.01[农业科学—作物学]