低氘水对D-半乳糖致衰老模型小鼠抗氧化能力的影响  

Effect of deuterium depleted water on antioxidant abilities in aging mice induced by D-galactoe

作　　者：王洁[1] 丛峰松[2] 蔡东联[1] 曲丹[1] 林宁[1] 郑慧[1] 连璐[1] 

机构地区：[1]第二军医大学长海医院临床营养科,上海200433 [2]上海交通大学生命科学技术学院,200240

出　　处：《武警医学》2010年第5期404-406,410,共4页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的研究低氘水(deuterium depleted water,DDW)对D-半乳糖致衰老模型小鼠抗氧化能力的影响。方法将24只ICR小鼠分为正常对照组、衰老模型组、低氘水实验组;衰老模型组与低氘水实验组颈背部皮下注射D-半乳糖建立衰老模型;正常对照组、衰老模型组饮用普通水,低氘水实验组饮用低氘水。7周后获取小鼠心、脑、肝、血清等组织测定总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、Na^+-K^+-ATP酶、单胺氧化酶(MAO)的活力、丙二醛(MDA)含量的差异。结果低氘水实验组较衰老组小鼠血清中SOD活力增高,MDA降低;心肌组织中SOD活力增高,MAO活力、MDA含量降低;脑组织中Na^+-K^+-ATP酶活力、T-AOC均增高,MDA减少;肝脏组织中SOD、GSH-Px活力均增高,MAO活力、MDA含量降低。结论低氘水对于衰老模型小鼠抗氧化能力有一定正性调节作用。Objective To study the antioxidant effect of the deuterium depleted water on D - galactose induced aging mouse models. Methods Twenty-four male ICR mice were equally divided into three groups： normal control group, aging model group and experimental group. The models of mouse aging were developed by continual intraperitoneal injection of D - galactose. Mice in the ex- perimental group drank deuterium depleted water and the other two groups drank tap water. The content of malondialdehyde （MDA） and activity of superoxide dismutase （ SOD）, glutathione peroxidase （ GSH - Px）, T - AOC, and Na^＋-K^＋-ATP ase monoamine oxidase （MAO） in the serum, liver and myocardial tissues of the mice were measared after seven weeks. Results In the experimenal group, the levels of SOD, T - AOC, GSH - Px, and Na^＋-K^＋-ATP were significantly higher than those in D - galactose induced aging group. The MDA content and the activity of MAO were lower than those in the aging group（P 〈 0.05）. Conclusions The deuterium depleted water greatly improves the effect of antioxidation on the D - galactose induced aging mouse model.

关 键 词：低氘水 抗氧化 衰老小鼠 

分 类 号：R151[医药卫生—营养与食品卫生学]