游泳池水微生物标准检验方法的研究  

A study on the methods of Microbial Standard Examination of the Swimming-pool Water

作　　者：张淑兰[1] 韩秀媛[1] 路金爽[1] 

机构地区：[1]天津市卫生防病中心

出　　处：《中国卫生检验杂志》1999年第2期83-87,共5页Chinese Journal of Health Laboratory Technology

摘　　要：本文结合制定国家标准探讨了游泳池水细菌总数的测定所用培养基的成分，培养的温度、时间和ＴＴＣ对菌落计数的影响；探讨了游泳池水大肠菌群测定所用培养基的选择性和特异性以及保湿剂对大肠菌群计数的影响。试验结果证实：ＩＳＯ国际标准方法所用培养基活菌计数的检出率高于我国ＧＢ５７５０—８５２４％，２２℃培养７２ｈ检出率最高，是３７℃培养２４ｈ的１．７倍。ＩＳＯ９３０８—２滤膜法测定大肠菌群所用培养基阴性菌和阳性菌颜色分明易于辨认，分离培养基上阴性菌为绿色，阳性菌为黄色。保湿剂在ＩＳＯ规定的剂量范围内加与不加对大肠菌群计数无影响。In order to draw up the National Standard, in this paper we probe into the influence on the colony counting of the medium ingredient, the culture tempreture, culture time and TTC, required for the examination of the sum of the bacteria in the swimming-pool water. We also probe into the media's electivity and speciality used for the detection for coliform organisms in the swimming-pool water and Tergitol's influence on the enumeration of coliform organisms. The experiment result shows that the radio of ISO detection of the enumeration of the viable is 24 per cent above our country's GB 5750-85. The radio of detection is the highest when it is cultured at 22℃ for 72h, 1 7 times above incubation at 37℃ for 24h. The medium used in the membrane filtration method of detection for coliform organisms has good electivity, the negative bacteria and the positive have the different colors which are distinguished easily. On the elective media the negative shows green and the positive shows yellow. Within the scope of ISO method, whether add Tergitol into the medium or not has no effect on the enumeration of coliform organisms.

关 键 词：游泳池水 微生物 标准方法 细菌总数 大肠菌群 

分 类 号：R126.4[医药卫生—环境卫生学] R117[医药卫生—公共卫生与预防医学]