麻疹病毒CC47疫苗测序分析及与其他A基因型病毒株比较  

Sequence analysis of vaccine CC47 and comparison with other genotype A strains of Measles virus

作　　者：王静宇[1,2] 吴伟立[1] 姜君[1] 邓海君[1] 白春杰 陈唯军[1] 

机构地区：[1]中国科学研究院北京基因组研究所,北京101300 [2]中国科学院研究生院,北京100049 [3]江苏延申生物科技股份有限公司研发部,常州213022

出　　处：《黑龙江大学自然科学学报》2010年第3期375-385,402,共12页Journal of Natural Science of Heilongjiang University

基　　金：全军医药卫生科研资助项目(08G021);卫生部传染病重大专项资助项目(2008ZX10004-001)

摘　　要：对长春-47(CC47)麻疹病毒减毒活疫苗即CC47疫苗进行测序分析,并与其他A基因型病毒株进行了比较。通过RT-PCR法扩增了6个编码基因,用双脱氧链终止法对产物进行自动测序。对结果进行比较分析发现,CC47疫苗有13个特有的氨基酸改变分别位于N,P和L蛋白,可以看做是该疫苗株的"疫苗标签"。通过构建系统发生树,分析了11株麻疹病毒的亲缘关系。本研究完善了CC47疫苗基因组信息,提供了对麻疹流行进行监测和对病毒减毒基因学基础进行进一步研究的潜在目标。The coding region of Changchun - 47 （CC47） vaccine had been sequenced and compared with other genotype A strains to reveal some vaccine information and learn more about genetic basis between nucleotide substitution and attenuation. Six coding gene had been amplified using RT-PGR, and products been automatically sequenced using dideoxy chain-termination method. Comparative analysis demonstrated CC47 vaccine possessed 13 exclusive amino acid changes located in N, P and L proteins, which were regarded as ＂vaccine strain signature＂. By constructing phylogenetic trees, the genetic relationship of I I measles fected the information of coding regions of CC47 vaccine, and provided surveillance and further study for genetic basis of attenuation. virus had been analyzed. This study pera potential target for the epidemiological

关 键 词：麻疹病毒 长春-47株疫苗 测序分析 疫苗标签 减毒 

分 类 号：R373.1[医药卫生—病原生物学]