脂质体介导Bcl-2反义脱氧寡核苷酸对人卵巢癌细胞作用的研究  

Effect of Lipofectamine induced Bcl-2 antisense oligodeoxynucleotides on human ovarian cancer cells

作　　者：孟祥凯[1] 陈盼[1] 任常山[2] 

机构地区：[1]中国医科大学附属第一医院妇科,辽宁沈阳110001 [2]中国医科大学附属第一医院肿瘤研究所第二研究室,辽宁沈阳110001

出　　处：《中国现代医学杂志》2010年第9期1305-1308,1313,共5页China Journal of Modern Medicine

摘　　要：目的探讨bcl-2反义脱氧寡核苷酸体外转染卵巢癌细胞后,对癌细胞bcl-2基因表达调控作用、诱导其凋亡的作用以及对顺铂敏感性的影响。方法体外培养顺铂耐药的人卵巢上皮性癌细胞系CAOV3,以阳离子脂质体为载体,将bcl-2反义脱氧寡核苷酸片断作用于CAOV3细胞。应用逆转录聚合酶链反应(RT-PCR)技术检测bcl-2基因的表达;流式细胞仪(FACS)检测转染后CAOV3细胞凋亡的变化;四甲基偶氮唑蓝(MTT)比色试验检测转染后CAOV3细胞对顺铂敏感性的影响。结果脂质体介导的bcl-2反义脱氧寡核苷酸作用于CAOV3细胞后,bcl-2基因mRNA表达水平显著低于对照组(P<0.01),并可诱导卵巢癌细胞凋亡,增强卵巢癌细胞对顺铂的敏感性。结论脂质体介导的bcl-2反义脱氧寡核苷酸作用于CAOV3细胞,可下调bcl-2基因的表达,诱导卵巢癌细胞凋亡,并增强CAOV3细胞对顺铂的敏感性。【Objective】To investigate the effect of Lipofectamine induced Bcl-2 antisense oligodeoxynucleotides（L-ASODN） on down-regulating Bcl-2 gene expression,inducing apoptosis and increasing the sensitivity of cisdichlorodiamine platinum（DDP） in human ovarian cancer cell line CAOV3（DDP-resistanced cells） in vitro.【Methods】Human ovarian cancer cell lines CAOV3 were maintained in vitro.Expression of bcl-2 mRNA of CAOV3 was evaluated by reverse transcription polymerase chain reaction（RT-PCR）.The induction of apoptosis in cultured CAOV3 cells was detected by FCM.Cell growth experiments were determined by MTT.【Results】Antisense oligodexynucleotides down-regulated bcl-2 gene mRNA level,and induced a higher percentage of apoptosis in CAOV3 cells,and DDP reduced the rate of cells survival.【Conclution】L-ASODN inhibited bcl-2 gene expression,and induced the apoptosis of CAOV3 cells,and enhanced the anti-tumor effect of DDP.

关 键 词：卵巢癌 BCL-2 反义脱氧寡核苷酸 

分 类 号：R737.31[医药卫生—肿瘤]