组蛋白去乙酰化酶抑制剂对人骨肉瘤细胞抑制增殖和诱导分化的研究  

Histone deacetylase inhibitor in inhibiting proliferation and inducing differentiation of human osteogenic sarcoma cells

作　　者：丁凡[1] 邵增务[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022

出　　处：《中国矫形外科杂志》2010年第13期1111-1114,共4页Orthopedic Journal of China

摘　　要：[目的]观察组蛋白去乙酰化酶抑制剂曲古抑菌素A(TSA)对人骨肉瘤MG-63细胞增殖和分化的影响。[方法]不同浓度的TSA作用MG-63细胞,采用噻唑蓝(MTT)法、倒置相差显微镜观察药物对细胞生长的影响并绘制细胞生长曲线;软琼脂集落形成实验观察细胞生长和侵袭能力的变化;流式细胞仪测定细胞周期变化。[结果]TSA可明显抑制MG-63细胞增殖,并呈现剂量和时间的依赖性;细胞形态呈明显的良性分化;瘤细胞的软琼脂集落形成率明显降低;TSA能够延缓细胞周期G1-S进程,阻滞细胞于G1期,停留在G2/M期。[结论]TSA对MG-63细胞有明显的抑制增殖和诱导分化作用。[ Objective] To study the effect of histone deacetylase inhibitor- Trichostatin A （TSA） on proliferation and differentiation of human osteogenic sarcoma cell. [ Methods] Human osteogenic sarcoma cell line MG-63 was treated in vitro with various concentrations of TSA, a potent and specific histone deacetylase inhibitor. Proliferation suppression was observed by MTT method and inverted microcopy before and after TSA treatment, and the cell growth curve was obtained. The cell growth and invasion ability were measured with the colony -formation rate in soft agar test. Flow cytometry was used to investigate the cell cycle. [ Results ] TSA significantly inhibited the proliferation of the human osteogenic sarcoma cell in a dose - dependent and time - dependent manner. The cell experienced benigh morphological differentiation. The colony - formation rate in semi - solid agar was significantly decreased （P 〈0. 01 ） . TSA delayed cell cycle G1 - S progression and induced a G1 cell cycle arrest. TSA can arrest the cells of cultured MG - 63 cells at G2/M phase. [ Conclusion] TSA can inhibit the proliferation of osteogenic sarcoma cell and lead to benign differentiation.

关 键 词：骨肉瘤 组蛋白去乙酰化酶 曲古抑菌素A 分化 

分 类 号：R738.1[医药卫生—肿瘤]