阻断孤啡肽受体对大鼠局灶性脑缺血损伤的保护作用  

The protection of orphanin receptor blockade on focal cerebral ischemia in rats

作　　者：王高翔[1] 刘文博[2] 孙绪德[1] 姚立农[1] 柴伟[1] 

机构地区：[1]第四军医大学唐都医院麻醉科,西安市710038 [2]第四军医大学神经外科

出　　处：《临床麻醉学杂志》2010年第3期239-241,共3页Journal of Clinical Anesthesiology

摘　　要：目的研究侧脑室给予孤啡肽受体(ORL-1)阻断药对大鼠脑缺血-再灌注损伤的影响。方法雄性SD大鼠55只,随机分为三个不同剂量的给药组(T1、T2、T3组)和假手术组(S组)、对照组(C组),每组11只。给药组和C组都采用线栓法阻断大脑中动脉模型(MCAO),缺血2h后恢复再灌注。给药组于手术前45min右侧脑室分别给予ORL-1阻断药Nphel0.03、0.3、3nmol,剂量10μl。每组大鼠随机分为两部分,其中8只在恢复再灌后24h行行为学评分(NDS),后断头取脑行TTC染色,计算脑梗死容积。3只在恢复再灌注后24h断头分离右侧海马组织,Western blot法分析Caspase-3蛋白表达。结果与C组相比,T3组的NDS明显提高(P<0.05),T2、T3组的脑梗死容积明显减小(P<0.05);T2、T3组在缺血-再灌注后24h Caspase-3蛋白表达明显降低。结论阻断ORL-1能剂量依赖性减轻脑缺血-再灌注损伤,其机制可能与阻断神经元凋亡有关。Objective To investigate the effect of intracerebroventricular administration of orphanin receptor antaonist Nphe on cerebral ischemia-reperfusion（I-R） injury in rats.Methods Fifty-five male SD rats were divided randomly into 5 groups of sham operated（ group S,control（ group C）,Nphe 0.03 nmol treated（group T1）,0.3 nmol（group T2） and 3 nmol（group T3）.The I-R model was established by the ligation of middle cerebral artery（MCAO） and reperfused after occlusion for 2 hours in group C and Nphe groups.The rats in Nphe groups were intracerebroventricularly given Nphe at 45 min before MCAO surgery.Of each group,8 rats were taken for behavioral scoring and TTC staining at 24 h after MCAO surgery,and 3 rats for detecting Caspase-3 level in right hippocampus by Western blot at 24 h after MCAO surgery.Results The behavior scores were higher in group T2 than those in group C（P〈0.05）.The volume of cerebral infarction in groups of T2and T 3 were significantly decreased dose-dependently（P〈0.05）.administration of 0.3 nmol and 3 nmol Nphe1 significantly decreased Caspase-3 protein expression in the hippocampus after cerebral I-R.Conclusion Orphanin receptor antaonist can attenuate the injury of cerebral I-R dose-dependently,which may be associated with a decrease in neuron apoptosis.

关 键 词：孤啡肽 孤啡肽受体 脑缺血-再灌注损伤 CASPASE-3 

分 类 号：R741[医药卫生—神经病学与精神病学]