早期丰富环境对脑白质损害大鼠海马生长相关蛋白43表达的影响  

Effects of Early Enriched Environment on Expressed of Growth Associated Protein 43 in Hippocampus of White Matter Damaged Neonatal Rats

作　　者：张征宇[1] 袁宝莉[2] 邓素娟[2] 

机构地区：[1]广州医学院组胚教研室,广州510185 [2]广州医学院附属第二医院儿科,广州510260

出　　处：《实用儿科临床杂志》2010年第13期1000-1002,共3页Journal of Applied Clinical Pediatrics

基　　金：广东省卫生厅资助项目(A2009271);广州市医药卫生科技项目(2008-YB-173)

摘　　要：目的探讨早期丰富环境对脑白质损害大鼠海马生长相关蛋白43(GAP-43)表达的影响。方法 2日龄新生大鼠29只,随机分为正常对照组(n=9)、干预组(n=10)及非干预组(n=10),后2组结扎右侧颈总动脉,术后休息2h,置密封舱中,吸入氧体积分数为60mL.L-1的氮氧混合气,制作新生大鼠脑白质损伤(WMD)模型。干预组采用早期触摸和丰富环境进行干预。早期触摸从损伤后第4天开始,连续实施7d。丰富环境干预从损伤后第11天开始,到第30天结束,总干预时间为27d。余2组正常饲养。干预结束后进行感觉运动功能检测,以判断干预效果。干预后第7天和第27天将大鼠处死,取其脑组织应用Westernblot方法检测海马GAP-43的表达。结果干预组感觉运动功能较非干预组显著改善(P<0.01)。非干预组和干预组海马GAP-43的表达在干预后7d时均高于正常对照组,在27d时干预组高于非干预组和正常对照组(Pa<0.01)。结论早期丰富环境可以上调脑白质损害大鼠海马GAP-43的表达,可能是其改善脑功能的机制之一。Objective To study the effects of early enriched environment on expression of growth associated protein 43 （ GAP - 43） in hippocampus of white matter damaged（WMD） neonatal rats. Methods Two days old postnatal SD rats （n ： 29） were collected and randomly divided into normal control group （n = 9）,intervention group （n = 10） and non-intervention group（ n = 10）. The latter 2 groups were subjected to WMD at first. The right common carotid artery was dissected and ligated,and after 2 hours rest,exposed to 60 mL · L^-1 oxygen 940 mL· L^-1 nitrogen gas mixture. After that, intervention group received the neonatal handling and was kept in an enriched environment for 27 days. Neonatal handling was applied from the 4^th day to the 10^th day after WMD. Then intervention group was put into enriched environment from the 11^th day to the 30th day after WMD. The other 2 groups were fed in normal environment. The abilities of sensorimotor function （ hanging test and inclined plane test） were observed after intervention, and Western blot was used to examine the expression of GAP -43 in hippocampus at the 7^th day and the 27^th day after intervention. Results The sensorimotor function abilities of intervention group were much higher than those in non - intervention group （P 〈 0.01 ）. Compared with that in control group, expressions of GAP -43 in intervention group and non -intervention group were higher on the 7th day after intervention （P 〈 0.01 ）. The expression of GAP-43 was upregulated on the 27^th day in intervention group （P 〈 0. 01 ）. Conclusions Early enriched environment can improve the motion ability of WMD neonatal rats,changing the expression of GAP - 43 in hippocampus is a possible cause.

关 键 词：脑白质损害 生长相关蛋白43 海马 丰富环境 大鼠 

分 类 号：R748[医药卫生—神经病学与精神病学]