三种尼氏小体染色方法对放疗后犬脊髓神经元显示效果的比较  

Neurons of beagle spinal cord showed by three stain methods after radiotherapy

作　　者：锡林宝勒日 徐万龙[1] 白靖平[1] 

机构地区：[1]新疆医科大学附属肿瘤医院骨与软组织肿瘤科,乌鲁木齐市830011

出　　处：《实用医学杂志》2010年第12期2112-2114,共3页The Journal of Practical Medicine

基　　金：新疆维吾尔自治区科技攻关(含重大专项)和重点项目(编号:200833116)

摘　　要：目的:探讨建立理想显示神经元微细结构的方法。方法:在10～14d内,对18只比格犬胸9～10椎体给予总剂量为60Gy的放疗。放射治疗结束3个月后,将18只比格犬以随机数字表法随机分为A、B、C组,每组6只,各组犬活杀后取脊髓并行横断切片,A组采用苏木精-伊红染色,B组采用传统ToluidineBlue染色(甲苯胺蓝染色),C组采用改进的Toluidine Blue染色法显示神经元结构。结果:苏木精-伊红染色后神经元结构不清晰,尼氏小体模糊。传统Toluidine Blue染色后组织切片全部为蓝色,对比度较差。改进后的Toluidine Blue染色后,尼氏小体、胞核及轴突、树突都清晰可见,且视野红蓝颜色相匹配,清晰度好,分辨率高。经病理图像及分析系统统计,改进后Toluidine Blue染色后组脊髓神经元内尼氏小体表达量与其他两组有差异(P<0.05),结果优于其他两组。结论:光镜下,改进的Toluidine Blue染色法的组织切片分辨率、美观度、清晰度较苏木精-伊红染色和传统的Toluidine Blue染色法的更好,更能清楚显示神经元的结构特征。该技术灵敏、准确、重现性好,可应用于放疗对脊髓神经元损伤的研究。Objective To explore an ideal method of showing the histological structure of the neurons. Methods Radiotherapy was administered to the ninth and the tenth thoracic vertebrae bodies of 18 purebred beagles. The total doses of radiotherapy were 60 Gy in 10～14 days. After three months ,beagles were divided into 3 groups （A, B, C group） randomly. Every group has 6 beagles. Then the 18 beagles were sacrificed. Their spinal cords were isolated and transected. Spinal cords of A group were stained with thionin-eosin （H-E）, while B group with traditional Toluidine Blue and C group with improved Toluidine Blue to show neurons, respectively. Results The structure of neurons was not clear and Nissl bodies were blurred when neurons were stained with H-E. When biopsy was stained with traditional Toluidine Blue, the bi1opsy was blue, and the contrast degree was poor. Nissl bodies, nucleus, axons and dendrites were clearly seen when stained with improved Toluidine Blue. Blue and red matched in vision with good definition and resolution. After pathological image and analysis system counted, the expression of Nissl bodies in neurons of C group was higher than other two groups（ P 0.05）. Conclusions Biopsy stained with the improved Toluidine Blue technique had higher definition, resolution, and aesthetics degree than that of H-E and traditional Toluidine Blue. The improved Toluidine Blue technique, which was more sensitive, accurate, and reappearing, could be used to research of spinal cord neurons damage resulted from radiotherapy.

关 键 词：神经元 尼氏小体 染剂 染色法 组织学技术 

分 类 号：R73-3[医药卫生—肿瘤]