黄铜矿表面生物吸附量的测定条件  

Conditions for Biosorption Measuring on Chalcopyrite Surface

作　　者：潘颢丹[1] 杨洪英[1] 陈世栋[1] 

机构地区：[1]东北大学材料与冶金学院,辽宁沈阳110004

出　　处：《东北大学学报（自然科学版）》2010年第7期999-1002,共4页Journal of Northeastern University(Natural Science)

基　　金：国家自然科学基金资助项目(50674029;50874030);国家高技术研究发展计划项目(2006AA06Z127);教育部高等学校博士学科点专项科研基金资助项目(20060145015);国家科技支撑计划项目(2008BAB34B01)

摘　　要：采用茚三酮比色法测定了在细菌浸矿的过程中,黄铜矿矿物表面吸附细菌细胞裂解液的吸光度,通过对测量波长、裂解时间、溶液pH值、加热时间、冷却时间等影响吸光度因素的实验研究,得出了黄铜矿表面细菌细胞裂解液吸光度的最佳测定条件为:波长560nm,裂解时间25min,溶液pH值7.0,加热时间15min,冷却时间6min.在最佳条件下绘制出了吸光度与矿物表面细菌吸附量之间关系的标准曲线.研究表明细菌裂解液的吸光值与细菌的浓度成正比.The ninhydrin colorimetry was used to measure the absorbance of the bacterial cell lysis solution adsorbed on the surface of chalcopyrite during bacterial leaching. The influencing factors on the absorbance, such as measuring wavelength, lysing time, pH value of the solution, heating time and cooling time were investigated experimentally to get the optimal measuring conditions for the absorbance of the lysis solution, i.e. , wavelength 560 nm, lysing time 25 min, pH value 7. 0, heating time 15 min and cooling time 6 min. A standard curve showing the relationship between the absorbance and bacteria adsorption under optimal conditions was therefore given. It was found that the bacterial concentration is directly proportional to the absorbance of the lysis solution.

关 键 词：生物吸附 茚三酮比色法 黄铜矿 细菌 矿物表面 

分 类 号：TF111.3[冶金工程—冶金物理化学]